PXD013617

PXD013617 is an original dataset announced via ProteomeXchange.

Title	Characterization under quasi-native conditions of the capsanthin/capsorubin synthase from Capsicum annuum L.
Description	Chromoplasts are typical plastids of fruits and flowers, deriving from chloroplasts through complex processes of re-organisation and recycling. Since this transition leads to the production of reactive species, chromoplasts are characteristic sites for biosynthesis and accumulation of carotenoids and other antioxidants. Here, we have analysed the chromoplast membranes from Capsicum annuum L. fruits, finding a significant expression of the capsanthin/capsorubin synthase. This enzyme was isolated by a very mild procedure allowing its analyses under quasi-native conditions. The isolated complex appeared as a red coloured homo-trimer, suggesting the retention of at least one of the typical carotenoids from C. annuum. Moreover, the protein complex was co-purified with a non-proteinaceous fraction of carotenoid aggregates carrying a high molecular weight and separable only by Size Exclusion Chromatography. This last finding suggested a relationship between the carotenoids synthesis on chromoplast membranes and the presence of organised carotenoids aggregates typical for chromoplasts. Further MS analyses also provided important hints on the interactome network associated to the capsanthin/capsorubin synthase, confirming its functional relevance during ripening. Results are discussed in the frame of the primary role played by carotenoids in quenching the growing oxidative stress during fruits ripening.
HostingRepository	PRIDE
AnnounceDate	2019-09-16
AnnouncementXML	Submission_2019-09-16_00:36:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joanna Kirkpatrick
SpeciesList	scientific name: Capsicum annuum; NCBI TaxID: 4072;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-24 03:43:40	ID requested
⏵ 1	2019-09-16 00:36:04	announced

Piano D, Cocco E, Guadalupi G, Kalaji HM, Kirkpatrick J, Farci D, Characterization under quasi-native conditions of the capsanthin/capsorubin synthase from Capsicum annuum L. Plant Physiol Biochem, 143():165-175(2019) [pubmed]

submitter keyword: Capsanthin/capsorubin synthase, Carotenoids, Chromoplast, Fruit ripening, Membrane protein complex, Oxidative stress, Red bell pepper

Domenica Farci
contact affiliation	Department of Plant Physiology Laboratory of Photobiology and Plant Physiology Faculty of Agriculture and Biology Warsaw University of Life Sciences Nowoursynowska Str. 159 02776 Warsaw Poland
contact email	domenica_farci@sggw.pl
lab head
Joanna Kirkpatrick
contact affiliation	Leibniz Institute on Aging - FLI
contact email	joanna.kirkpatrick@leibniz-fli.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013617
     1. Label: PRIDE project
     2. Name: Characterization under quasi-native conditions of the capsanthin/capsorubin synthase from Capsicum annuum L.