PXD013614
PXD013614 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Label-free quantification of HEK293T-BTZ |
| Description | HEK293T cells were untreated or treated with 50 μM GlcNAz with DMSO for 48 hr (control), or 7.5 nM BTZ alone for 48 hr (BTZ), or 10 nM BTZ for 48hr and 20 μM OSMI-1 for 24 hr (BTZ+OSMI-1). Cell lysis and Click chemistry-based purification were performed using Click-iT protein enrichment kit following to manufacturer’s protocols with some modifications. Proteins on the beads were digested by adding trypsin. The digests were desalted using GL-Tip SDB (GL Sciences), and the eluates were evaporated in a SpeedVac concentrator. LC-MS/MS analysis of the resultant peptides was performed on an EASY-nLC 1200 UHPLC connected to a Q Exactive Plus mass spectrometer through a nanoelectrospray ion source (Thermo Fisher Scientific). The peptides were separated on a 75 μm inner diameter × 150 mm C18 reversed phase column (Nikkyo Technos) with a linear gradient from 4–28% acetonitrile (ACN) for min 0–100 followed by an increase to 80% ACN during min 100–110. Dynamic exclusion was set to 10 sec. Raw data were analyzed directly against the SwissProt database restricted to Homo sapiens using Proteome Discoverer version 2.2 |
| HostingRepository | jPOST |
| AnnounceDate | 2023-04-08 |
| AnnouncementXML | Submission_2023-04-07_08:00:05.893.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hidetaka Kosako |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue |
| Instrument | instrument |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-04-24 01:35:08 | ID requested | |
| ⏵ 1 | 2023-04-07 08:00:06 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: LFQ, HEK293T, BTZ, OSMI-1 |
Contact List
| Hidetaka Kosako | |
|---|---|
| lab head | |
| Hidetaka Kosako | |
| contact affiliation | Tokushima University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000591/ |
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