PXD013613

PXD013613 is an original dataset announced via ProteomeXchange.

Title	Deoxynivalenol and human epidermal cells: proteomic profiling indicates alterations of cell membrane integrity and barrier function
Description	The possibility to combine untargeted proteomic workflows to more classical experimental approaches is continuously opening new insights in all branches of biological sciences. Deoxynivalenol (vomitoxin, DON) is a secondary metabolite produced by Fusarium spp. fungi and it is one of the most recurrent mycotoxins worldwide. DON is known to inhibit protein synthesis and as such to interact with the different cell types in multiple and complex ways. For the purpose of this study epidermoid squamous cell carcinoma cells A431 and primary human HEKn cells were incubated with DON for 24h and toxin dependent alteration of the proteome profile was observed in the nuclear and cytoplasmic fraction. In A431 cells, DON significantly down-regulated ribosomal proteins, as well as mitochondrial respiratory chain elements (OXPHOS regulation) and transport proteins (TOMM22; TOMM40; TOMM70A). In line with the impairment of the mitochondrial function, altered metabolic capability was observed, with particular target of the lipid synthesis machinery. Effect of the mycotoxin on cell membrane was verified by confocal microscopy (morphology) and by membrane fluidity measures (biophysical properties). The toxicological relevance of these findings was independently confirmed with the primary human keratinocytes HEKn.
HostingRepository	PRIDE
AnnounceDate	2021-05-03
AnnouncementXML	Submission_2021-05-02_23:23:49.162.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013613
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-24 01:23:10	ID requested
⏵ 1	2021-05-02 23:23:50	announced

Del Favero G, Janker L, Neuditschko B, Hohenbichler J, Kiss E, Woelflingseder L, Gerner C, Marko D, Exploring the dermotoxicity of the mycotoxin deoxynivalenol: combined morphologic and proteomic profiling of human epidermal cells reveals alteration of lipid biosynthesis machinery and membrane structural integrity relevant for skin barrier function. Arch Toxicol, 95(6):2201-2221(2021) [pubmed]

submitter keyword: Deoxynivalenol (DON, vomitoxin)

human epidermal keratinocytes (HEKn), lipid synthesis machinery

Christopher Gerner
contact affiliation	University of Vienna Faculty of Chemistry Department of Analytical Chemistry
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013613
     1. Label: PRIDE project
     2. Name: Deoxynivalenol and human epidermal cells: proteomic profiling indicates alterations of cell membrane integrity and barrier function