PXD013610

PXD013610 is an original dataset announced via ProteomeXchange.

Title	Resolving oxidative damage to methionine by an unexpected membrane-associated stereoselective reductase discovered using chiral fluorescent probes
Description	Non-enzymatic oxidative processes in living organisms are one of the inevitable consequences of respiration and environmental conditions. They can lead to the formation of two stereoisomers (R and S) of methionine sulfoxide, and the redox balance between methionine and methionine sulfoxide in proteins has profound functional consequences. Methionine oxidation can be reverted enzymatically by methionine sulfoxide reductases (Msrs). The two enzyme classes known to fulfil this role are MsrA (reducing (S)-sulfoxides), and MsrB (reducing (R)-sulfoxides). They are strictly stereoselective and conserved throughout the tree of life. Under stress conditions such as stationary phase and nutrient starvation, E.coli upregulates expression of MsrA but similar effect has not been described for MsrB, raising a conundrum of the pathway enabling reduction of the (R)-isomer of methionine sulfoxide in these conditions. Using the recently developed chiral fluorescent probes Sulfox-1 we show that in stationary phase stressed E. coli, MsrA does have a stereocomplementary, (R)-sulfoxide-reducing counterpart. However, this activity is not provided by MsrB as expected, but instead by the DMSO reductase complex DmsABC, widely conserved in bacteria. This finding reveals an unexpected diversity in the metabolic enzymes of redox regulation concerning methionine, which should be taken into account in any antibacterial strategies exploiting oxidative stress.
HostingRepository	PRIDE
AnnounceDate	2019-06-12
AnnouncementXML	Submission_2019-06-13_07:05:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-23 07:52:26	ID requested
1	2019-06-12 06:12:47	announced
⏵ 2	2019-06-13 07:05:43	announced	Updated publication reference for PubMed record(s): 31166082.

Makukhin N, Havelka V, Pol, á, chov, á E, Ramp, í, rov, á P, Tarallo V, Strisovsky K, M, í, š, ek J, Resolving oxidative damage to methionine by an unexpected membrane-associated stereoselective reductase discovered using chiral fluorescent probes. FEBS J, 286(20):4024-4035(2019) [pubmed]

submitter keyword: methionine sulfoxide, E.coli , oxidative damage

Kvido Strisovsky
contact affiliation	Institute of Organic Chemistry and Biochemistry, Czech Academy of Science, Flemingovo n.2, 16000 Prague, Czech Republic
contact email	kvido.strisovsky@uochb.cas.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013610
     1. Label: PRIDE project
     2. Name: Resolving oxidative damage to methionine by an unexpected membrane-associated stereoselective reductase discovered using chiral fluorescent probes