PXD013608

PXD013608 is an original dataset announced via ProteomeXchange.

Title	NIST Candidate RM 8461 Human Liver for Proteomics
Description	Advances in mass spectrometry (MS) instrumentation resulting in higher mass resolution and higher duty cycles have allowed deeper coverages of proteomes. The development of candidate RM 8461 Human Liver for Proteomics was undertaken to provide the community with a sample with suitable complexity for method development and validation, instrument and data validation, and determining identification QC thresholds. Herein we describe a comparison of methods and homogeneity assessment in which candidate RM 8461 Human Liver for Proteomics is solubilized, the proteins reduced, alkylated, digested and the tryptic peptides and their modifications are identified by bottom up LC/MS/MS to assess deep (e.g. thousands of identifications) protein coverage.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:26:30.764.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013608
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	W. Clay Davis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-23 06:44:45	ID requested
⏵ 1	2024-10-07 10:26:31	announced

10.1038/s41597-019-0336-7;

10.6019/PXD013608;

Davis WC, Kilpatrick LE, Ellisor DL, Neely BA, Characterization of a human liver reference material fit for proteomics applications. Sci Data, 6(1):324(2019) [pubmed]

submitter keyword: Human, QC Material, LC-MSMS, Reference Material, NIST, Liver, Orbitrap

Clay Davis
contact affiliation	National Institute of Standards and Technology Chemical Sciences Division Charleston, SC, USA
contact email	clay.davis@nist.gov
lab head
W. Clay Davis
contact affiliation	National Institute of Standards and Technology
contact email	clay.davis@nist.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD013608

PRIDE project URI

• PRIDE
  1. PXD013608
     1. Label: PRIDE project
     2. Name: NIST Candidate RM 8461 Human Liver for Proteomics