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PXD013601

PXD013601 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of MARCH2 E3 ligase-substrates using proximity-dependent biotin labeling method
DescriptionMARCH2 is known to be involved in intracellular vesicular trafficking. To identify its substrates, we have recently developed a method based on proximity-dependent biotin labelling. In this protocol, the MARCH2 ubiquitin ligase of interest is expressed as a fusion to Escherichia coli biotin ligase BirA together with a biotin acceptor peptide(AP)-tagged ubiquitin. The BirA-directed biotin labeling of AP depends on the proximity of the two fusion proteins in the cell, which leads to preferential labeling of ubiquitinated E3 substrates. In this study, we applied this procedure to MARCH2 and identified its substrates.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:55:38.026.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWonjin Yoo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitinylated lysine; biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-23 05:43:27ID requested
12019-06-06 00:43:50announced
22024-10-22 04:55:38announced2024-10-22: Updated project metadata.
Publication List
10.1074/jbc.ra119.007435;
Yoo W, Cho EB, Kim S, Yoon JB, The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins. J Biol Chem, 294(28):10900-10912(2019) [pubmed]
Keyword List
submitter keyword: MARCH2, secretory proteins, E3 ligase
Contact List
Jong-Bok Yoon
contact affiliationDepartment of Biochemistry, College of Life Science & Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-Gu, Seoul 120-749, Korea. Tel.: +82-2-2123-2704; Fax: 82-2-362-9897.
contact emailyoonj@yonsei.ac.kr
lab head
Wonjin Yoo
contact affiliationYonsei university
contact emailyu.stardust1234@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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