PXD013601 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of MARCH2 E3 ligase-substrates using proximity-dependent biotin labeling method |
Description | MARCH2 is known to be involved in intracellular vesicular trafficking. To identify its substrates, we have recently developed a method based on proximity-dependent biotin labelling. In this protocol, the MARCH2 ubiquitin ligase of interest is expressed as a fusion to Escherichia coli biotin ligase BirA together with a biotin acceptor peptide(AP)-tagged ubiquitin. The BirA-directed biotin labeling of AP depends on the proximity of the two fusion proteins in the cell, which leads to preferential labeling of ubiquitinated E3 substrates. In this study, we applied this procedure to MARCH2 and identified its substrates. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:55:38.026.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wonjin Yoo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitinylated lysine; biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-23 05:43:27 | ID requested | |
1 | 2019-06-06 00:43:50 | announced | |
⏵ 2 | 2024-10-22 04:55:38 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/jbc.ra119.007435; |
Yoo W, Cho EB, Kim S, Yoon JB, The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins. J Biol Chem, 294(28):10900-10912(2019) [pubmed] |
Keyword List
submitter keyword: MARCH2, secretory proteins, E3 ligase |
Contact List
Jong-Bok Yoon |
contact affiliation | Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-Gu, Seoul 120-749, Korea. Tel.: +82-2-2123-2704; Fax: 82-2-362-9897. |
contact email | yoonj@yonsei.ac.kr |
lab head | |
Wonjin Yoo |
contact affiliation | Yonsei university |
contact email | yu.stardust1234@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013601
- Label: PRIDE project
- Name: Identification of MARCH2 E3 ligase-substrates using proximity-dependent biotin labeling method