PXD013601

PXD013601 is an original dataset announced via ProteomeXchange.

Title	Identification of MARCH2 E3 ligase-substrates using proximity-dependent biotin labeling method
Description	MARCH2 is known to be involved in intracellular vesicular trafficking. To identify its substrates, we have recently developed a method based on proximity-dependent biotin labelling. In this protocol, the MARCH2 ubiquitin ligase of interest is expressed as a fusion to Escherichia coli biotin ligase BirA together with a biotin acceptor peptide(AP)-tagged ubiquitin. The BirA-directed biotin labeling of AP depends on the proximity of the two fusion proteins in the cell, which leads to preferential labeling of ubiquitinated E3 substrates. In this study, we applied this procedure to MARCH2 and identified its substrates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:55:38.026.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wonjin Yoo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine; biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-23 05:43:27	ID requested
1	2019-06-06 00:43:50	announced
⏵ 2	2024-10-22 04:55:38	announced	2024-10-22: Updated project metadata.

10.1074/jbc.ra119.007435;

Yoo W, Cho EB, Kim S, Yoon JB, The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins. J Biol Chem, 294(28):10900-10912(2019) [pubmed]

submitter keyword: MARCH2, secretory proteins, E3 ligase

Jong-Bok Yoon
contact affiliation	Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-Gu, Seoul 120-749, Korea. Tel.: +82-2-2123-2704; Fax: 82-2-362-9897.
contact email	yoonj@yonsei.ac.kr
lab head
Wonjin Yoo
contact affiliation	Yonsei university
contact email	yu.stardust1234@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD013601

PRIDE project URI

• PRIDE
  1. PXD013601
     1. Label: PRIDE project
     2. Name: Identification of MARCH2 E3 ligase-substrates using proximity-dependent biotin labeling method