PXD013549

PXD013549 is an original dataset announced via ProteomeXchange.

Title	Cilia and the exocyst are involved in urinary extracellular vesicle production and content
Description	A recently proposed model of cell-cell communication, called “urocrine signaling”, hypothesizes that small (50-150 mm) extracellular vesicles (EVs) carry signals to “downstream” kidney tubule cells. We previously showed that the exocyst, a highly-conserved eight-protein (Exoc1-8) trafficking complex, is necessary for ciliogenesis, we show that the EV number released from MDCK cells was increased in EXOC5 OE, while it was decreased in Exoc5 KD, compared to control. To determine how Exoc5 changes the composition of EVs, we isolated EVs from Exoc5 control, KD, and OE MDCK cells, and subjected them mass spectrometry-based proteomic analysis to determine the protein composition of the EVs. By dendrogram, heat map, and principle component analysis, the protein content of the EVs was significantly different in Exoc5 KD, EXOC5 OE, and control MDCK cells. To confirm the proteomic results, we examined, by Western blot analysis, expression of the small guanosine triphosphate (GTP)-binding protein ADP-ribosylation factor (Arf6) and the epidermal growth factor receptor kinase substrate 8-like protein 2 (Eps8L2).
HostingRepository	PRIDE
AnnounceDate	2020-01-13
AnnouncementXML	Submission_2020-01-13_06:56:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Starr Hazard
SpeciesList	scientific name: Canis lupus familiaris; NCBI TaxID: 9615;
ModificationList	(R)-5-oxo-1,4-tetrahydrothiazine-3-carboxylic acid; methylated cysteine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-18 02:41:36	ID requested
⏵ 1	2020-01-13 06:56:03	announced

Zuo X, Kwon SH, Janech MG, Dang Y, Lauzon SD, Fogelgren B, Polgar N, Lipschutz JH, Primary cilia and the exocyst are linked to urinary extracellular vesicle production and content. J Biol Chem, 294(50):19099-19110(2019) [pubmed]

ProteomeXchange project tag: Bioinformatics Infrastructure for Life Sciences (BILS) network (Sweden)

submitter keyword: extracellular vesicle, exocyst, GTPase, protein trafficking

Josh Lipschutz, M.D.
contact affiliation	Renal Division Director Professor of Medicine and Arthur V. Williams Chair Medical University of South Carolina
contact email	Lipschut@musc.edu
lab head
Starr Hazard
contact affiliation	Medical University of South Carolina
contact email	hazards@musc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD013549

PRIDE project URI

• PRIDE
  1. PXD013549
     1. Label: PRIDE project
     2. Name: Cilia and the exocyst are involved in urinary extracellular vesicle production and content