PXD013542

PXD013542 is an original dataset announced via ProteomeXchange.

Title	BMP2K is an inhibitor of erythroid differentiation that restricts endocytosis and SEC16A-dependent autophagy
Description	Coordination of membrane-related processes inside the cells is facilitated by proteins regulating multiple vesicular trafficking pathways. Bone morphogenetic protein 2-inducible kinase (BMP2K) is a poorly studied ubiquitous protein with the highest expression in the erythroid lineage. We hypothesized that this kinase, potentially implicated in endocytosis and autophagy, could integrate various trafficking routes. By proximity biotinylation (BioID), we revealed differential association of two BMP2K splicing variants with regulators of clathrin-mediated endocytosis (CME) and with components of early secretory pathway (ESP). We found the longer variant (BMP2K-L) to interact with markers of clathrin-coated pits, REPS1 and phosphorylated μ2 adaptin, and the shorter variant (BMP2K-S) to associate with early secretory compartments positive for ARFGAP1 and SEC16A proteins. Splicing variant-specific RNAi approach in K562 erythroleukemia cells showed that BMP2K-L controls μ2 phosphorylation while BMP2K-S post-transcriptionally maintains SEC16A protein levels. Depleting all BMP2K splicing variants promoted autophagic degradation via a functional crosstalk with SEC16A, enhanced transferrin endocytosis, and erythroid differentiation. We propose that, owing to alternative splicing, BMP2K gene products restrict erythroid differentiation coordinating endocytosis and SEC16A-dependent autophagy.
HostingRepository	PRIDE
AnnounceDate	2020-09-14
AnnouncementXML	Submission_2020-09-13_22:24:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jaroslaw Cendrowski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-17 05:51:01	ID requested
1	2019-07-31 05:38:26	announced
⏵ 2	2020-09-13 22:24:46	announced	2020-09-14: Updated publication reference for PubMed record(s): 32795391.

Cendrowski J, Kaczmarek M, Mazur M, Kuzmicz-Kowalska K, Jastrzebski K, Brewinska-Olchowik M, Kominek A, Piwocka K, Miaczynska M, Splicing variation of BMP2K balances abundance of COPII assemblies and autophagic degradation in erythroid cells. Elife, 9():(2020) [pubmed]

submitter keyword: HEK293, BioID, LC-MS/MS

Marta Miaczynska
contact affiliation	Laboratory of Cell Biology, International Institute of Molecular and Cell Biology, Warsaw, Poland
contact email	miaczynska@iimcb.gov.pl
lab head
Jaroslaw Cendrowski
contact affiliation	International Institute of Molecular and Cell Biology in Warsaw, Poland
contact email	jcendrowski@iimcb.gov.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013542
     1. Label: PRIDE project
     2. Name: BMP2K is an inhibitor of erythroid differentiation that restricts endocytosis and SEC16A-dependent autophagy