PXD013532

PXD013532 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae
Description	Mycetoma is a neglected chronic and granulomatous infection primarily associated with the fungal pathogen Madurella mycetomatis. Infection is characterised by the formation of fungal grains inside the infected tissue which commonly result in severe deformity and disability. Currently the biochemical processes and interactions between host and pathogen which result in grain formation are unknown. Furthermore, the infection process in mammals takes months to fully develop. In order to unravel these processes Galleria mellonella larvae were infected with M. mycetomatis and hyphae and grain formation, survival, fungal burden and proteomic responses of larvae were monitored for 10 days. At 24 h post infection proteins indicative of muscle invasion and humoral immune response activation were enriched in infected larval hemolymph. By 72 h immune related hdd11 was increased 337 fold, heat shock proteins 90 was increased 40 fold and glutathione-S-transferase was increased 25 fold. By 7 days post infection proteins which were associated with grain formation (hdd11 [533 fold], hemocentin [54 fold]) and a range of antimicrobial peptides were enriched. During the 7 day period a variety of proteins were decreased in infected hemolymph (e.g. hexamerin, apolipophorin and cationic peptide CP8). This data also identified 75 M. mycetomatis proteins released into hemolymph during infection. Proteins were also extracted from M. mycetomatis grains taken from larvae infected for 24, 72 and 7 days. These proteins give an insight into the interactions between the larval immune response and M. mycetomatis at the cellular levels during infection. These results identify similarities between the infection processes of M. mycetomatis in G. mellonella larvae and in humans and identify novel proteins from M. mycetomatis which may play a crucial role in grain development.
HostingRepository	PRIDE
AnnounceDate	2020-03-19
AnnouncementXML	Submission_2020-04-13_22:54:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerard Sheehan
SpeciesList	scientific name: Madurella mycetomatis; NCBI TaxID: 100816; scientific name: Galleria mellonella; NCBI TaxID: 7137;
ModificationList	iodoacetic acid derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-16 09:02:53	ID requested
1	2020-03-19 08:31:03	announced
⏵ 2	2020-04-13 22:54:49	announced	2020-04-14: Updated publication reference for PubMed record(s): 32267851.

Sheehan G, Konings M, Lim W, Fahal A, Kavanagh K, van de Sande WWJ, Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. PLoS Negl Trop Dis, 14(4):e0008190(2020) [pubmed]

submitter keyword: Madurella

Galleria, Infection, Immunity, Mini-model, in vivo screening, Proteomics, Grain

Kevin Kavanagh
contact affiliation	Medical Mycology Laboratory, Department of Biology, Maynooth University, Co. Kildare, Ireland.
contact email	kevin.kavanagh@mu.ie
lab head
Gerard Sheehan
contact affiliation	Maynooth University
contact email	gerard.sheehan.2013@mumail.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD013532

PRIDE project URI

• PRIDE
  1. PXD013532
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae