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PXD013525

PXD013525 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCRK2-mediated control of ROS production by phosphorylation of the RBOHD C-terminus in Arabidopsis
DescriptionReactive oxygen species (ROS) are important messengers in eukaryotic organisms. Extracellular ROS production by NADPH oxidases in plants is triggered by receptor-like protein kinase (RLK)-dependent signaling networks. This ROS production is tightly controlled by multiple mechanisms including phosphorylation by different kinases. Here we show that the cysteine-rich RLK CRK2 exists in a preformed complex with the NADPH oxidase RBOHD at the plasma membrane. Functional CRK2 is required for the full pathogen-induced ROS burst and consequently the crk2 mutant is impaired in defense against the bacterial pathogen Pseudomonas syringae. We identified phosphorylation sites in the C-terminal region of RBOHD and mutations of the phosphorylation sites modulate ROS production in response to biotic stimuli. Our work demonstrates that CRK2 occupies a central role in controlling ROS production and highlights that regulation of NADPH oxidase activity by phosphorylation of the C-terminal region is an ancient mechanism which is conserved between animals and plants.
HostingRepositoryPanoramaPublic
AnnounceDate2020-03-27
AnnouncementXMLSubmission_2020-03-27_17:15:27.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSara Stolze
SpeciesList scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationListPhospho; Carbamidomethyl
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-16 06:21:12ID requested
12020-03-27 17:15:28announced
Publication List
Kimura S, Hunter K, Vaahtera L, Tran HC, Citterico M, Vaattovaara A, Rokka A, Stolze SC, Harzen A, Mei, ß, ner L, Wilkens MMT, Hamann T, Toyota M, Nakagami H, Wrzaczek M, CRK2 and C-terminal Phosphorylation of NADPH Oxidase RBOHD Regulate Reactive Oxygen Species Production in Arabidopsis. Plant Cell, 32(4):1063-1080(2020) [pubmed]
Keyword List
submitter keyword: Phosphoproteomics, Reactive-oxygen species, receptor-like protein kinases, Arabidopsis thaliana
Contact List
Hirofumi Nakagami
contact affiliationBasic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact emailnakagami@mpipz.mpg.de
lab head
Sara Stolze
contact affiliationMax Planck Institute for Plant Breeding Research, Plant Proteomics and Mass Spectrometry Group
contact emailstolze@mpipz.mpg.de
dataset submitter
Full Dataset Link List
Panorama Public dataset URI