PXD013523

PXD013523 is an original dataset announced via ProteomeXchange.

Title	Papillary renal cell carcinomas rewire glutathione metabolism
Description	Papillary renal cell carcinoma (pRCC) is a malignant kidney cancer with a prevalence of 7-20% of all renal cell tumors. Proteome and metabolome profiles of 19 pRCC and patient-matched healthy kidney controls and transcriptome data derived from TCGA were used to elucidate the regulation of metabolic pathways and underlying molecular mechanisms. Glutathione (GSH), a main reactive oxygen species (ROS) scavenger, was tremendously increased in pRCC type I, II, and metastatic type II and can be regarded as a new hallmark in this malignancy. Isotope tracing revealed an increased de novo synthesis rate of GSH and a glutamine addiction in pRCC derived cell lines. Furthermore, a rewiring of the main pathways involved in ATP and glucose synthesis was observed on the protein level: the abundance of enzymes involved in gluconeogenesis and of the respiratory chain was found to be significantly reduced in pRCC. In contrast, transcripts encoding for the respiratory chain were not regulated, which prompts for non-genetic profiling. The main molecular characteristics of pRCC are the increased GSH synthesis to cope with ROS stress, the deficient anabolic glucose synthesis, and the compromised oxidative phosphorylation, which could be exploited in novel anti-cancer strategies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:56:11.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ayham Alahmad
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-16 02:54:55	ID requested
1	2019-09-06 04:44:19	announced
2	2019-10-10 09:02:02	announced	2019-10-10: Updated project metadata.
⏵ 3	2024-10-22 04:56:12	announced	2024-10-22: Updated project metadata.

10.3390/cancers11091298;

Al Ahmad A, Paffrath V, Clima R, Busch JF, Rabien A, Kilic E, Villegas S, Timmermann B, Attimonelli M, Jung K, Meierhofer D, Papillary Renal Cell Carcinomas Rewire Glutathione Metabolism and Are Deficient in Both Anabolic Glucose Synthesis and Oxidative Phosphorylation. Cancers (Basel), 11(9):(2019) [pubmed]

submitter keyword: metabolome Profiling

glutathione metabolism

metabolic reprogramming

David Meierhofer
contact affiliation	Mass Spectrometry Facility Max Planck Institute for Molecular Genetics
contact email	Meierhof@molgen.mpg.de
lab head
Ayham Alahmad
contact affiliation	Max Planck Institute for Molecular Genetics
contact email	alahmad@molgen.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD013523

PRIDE project URI

• PRIDE
  1. PXD013523
     1. Label: PRIDE project
     2. Name: Papillary renal cell carcinomas rewire glutathione metabolism