PXD013508

PXD013508 is an original dataset announced via ProteomeXchange.

Title	Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression
Description	Base excision repair (BER) initiated by alkyladenine DNA glycosylase (AAG; aka MPG) is essential for removal of aberrantly methylated DNA bases. Genome instability and accumulation of aberrant bases accompany multiple diseases including cancer and neurological disorders. While BER is well studied on naked DNA, it is currently unclear how BER efficiently operates on chromatin. Here we show that AAG binds to chromatin and forms a complex with active RNA polymerase (pol) II. This occurs through direct binding to Elongator and results in co-regulation of gene expression. Interestingly, endogenous aberrantly methylated bases accumulate at 3’end of co-regulated genes, in regions enriched for Elongator, active RNA pol II, and BER enzymes AAG and APE1. Active transcription and functional Elongator are further vital to ensure efficient BER by promoting AAG and APE1 chromatin occupancy. Our findings indicate that AAG needs to associate with transcription elongation to maintain genome stability, concurrently coordinating repair with gene expression.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:05:15.133.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Animesh Sharma
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-15 07:27:20	ID requested
1	2020-05-26 14:02:19	announced
⏵ 2	2024-10-22 05:05:17	announced	2024-10-22: Updated project metadata.

Montaldo NP, Bordin DL, Brambilla A, R, ö, singer M, Fordyce Martin SL, Bj, ø, r, å, s K Ø, Bradamante S, Aas PA, Furrer A, Olsen LC, Kunath N, Otterlei M, S, æ, trom P, Bj, ø, r, å, s M, Samson LD, van Loon B, Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression. Nat Commun, 10(1):5460(2019) [pubmed]

10.1038/s41467-019-13394-w;

submitter keyword: Cancer cachexia

Skeletal muscle wasting

Muscle atrophy

Endurance exercise

Aerobic exercise

Barbara van Loon
contact affiliation	Associate Professor Department of Clinical and Molecular Medicine Faculty of Medicine and Health Sciences +47 72824684 Laboratoriesenteret, Øya
contact email	barbara.v.loon@ntnu.no
lab head
Animesh Sharma
contact affiliation	Engineer at NTNU, Norway
contact email	sharma.animesh@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD013508

PRIDE project URI

• PRIDE
  1. PXD013508
     1. Label: PRIDE project
     2. Name: Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression