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PXD013508

PXD013508 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAlkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression
DescriptionBase excision repair (BER) initiated by alkyladenine DNA glycosylase (AAG; aka MPG) is essential for removal of aberrantly methylated DNA bases. Genome instability and accumulation of aberrant bases accompany multiple diseases including cancer and neurological disorders. While BER is well studied on naked DNA, it is currently unclear how BER efficiently operates on chromatin. Here we show that AAG binds to chromatin and forms a complex with active RNA polymerase (pol) II. This occurs through direct binding to Elongator and results in co-regulation of gene expression. Interestingly, endogenous aberrantly methylated bases accumulate at 3’end of co-regulated genes, in regions enriched for Elongator, active RNA pol II, and BER enzymes AAG and APE1. Active transcription and functional Elongator are further vital to ensure efficient BER by promoting AAG and APE1 chromatin occupancy. Our findings indicate that AAG needs to associate with transcription elongation to maintain genome stability, concurrently coordinating repair with gene expression.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:05:15.133.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnimesh Sharma
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; monohydroxylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-15 07:27:20ID requested
12020-05-26 14:02:19announced
22024-10-22 05:05:17announced2024-10-22: Updated project metadata.
Publication List
Montaldo NP, Bordin DL, Brambilla A, R, ö, singer M, Fordyce Martin SL, Bj, ø, r, å, s K Ø, Bradamante S, Aas PA, Furrer A, Olsen LC, Kunath N, Otterlei M, S, æ, trom P, Bj, ø, r, å, s M, Samson LD, van Loon B, Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression. Nat Commun, 10(1):5460(2019) [pubmed]
10.1038/s41467-019-13394-w;
Keyword List
submitter keyword: Cancer cachexia
Skeletal muscle wasting
Muscle atrophy
Endurance exercise
Aerobic exercise
Contact List
Barbara van Loon
contact affiliationAssociate Professor Department of Clinical and Molecular Medicine Faculty of Medicine and Health Sciences +47 72824684 Laboratoriesenteret, Øya
contact emailbarbara.v.loon@ntnu.no
lab head
Animesh Sharma
contact affiliationEngineer at NTNU, Norway
contact emailsharma.animesh@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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