PXD013506

PXD013506 is an original dataset announced via ProteomeXchange.

Title	Proteomic comparison of Prochlorococcus marinus ecotypes MED4, MIT9319 and SS120.
Description	Prochlorococcus marinus is a highly abundant picocyanobacterium in Earth’s oceans and therefore a significant contributor to global primary production. This organism exists as different ecotypes, each occupying particular environments in the euphotic zone that differ in both solar penetration and nutrient levels. The ecotypes analysed here were isolated from depths of 5 m (MED4), 135 m (MIT9313) and 120 m (SS120) and cultured at low illumination. MED4, adapted to high light levels closer to the surface, was compared at both low and high illumination. In contrast to other cyanobacteria such as Synechocystis with a dominance of photosystem I (PSI) over photosystem II (PSII) complexes in the thylakoid membranes, MED4 and MIT9313 showed about equal levels. In MED4, the relative levels were almost the same in both the high and low light cultures. SS120 thylakoids contained a lower cytochrome b6f content and around two-fold more PSII than PSI. Additionally a significantly higher abundance of light-harvesting Pcb proteins was found in SS120 than the other ecotypes. This proteomic comparison was employed in conjunction with thylakoid membrane AFM imaging to rationalize the strategies these ecotypes use to survive in the different oceanic environments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:26:02.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Prochlorococcus marinus str. MIT 9313; NCBI TaxID: 74547;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-15 07:25:44	ID requested
1	2021-09-08 10:00:59	announced
⏵ 2	2024-10-22 05:26:04	announced	2024-10-22: Updated project metadata.

10.1038/s41477-019-0475-z;

MacGregor-Chatwin C, Jackson PJ, Sener M, Chidgey JW, Hitchcock A, Qian P, Mayneord GE, Johnson MP, Luthey-Schulten Z, Dickman MJ, Scanlan DJ, Hunter CN, Membrane organization of photosystem I complexes in the most abundant phototroph on Earth. Nat Plants, 5(8):879-889(2019) [pubmed]

submitter keyword: Photosystem I,Cytochrome b6f,Prochlorococcus marinus, Pcb proteins, Photosystem II

Christopher Neil Hunter
contact affiliation	Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
contact email	c.n.hunter@sheffield.ac.uk
lab head
Philip Jackson
contact affiliation	School of Biosciences, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD013506

PRIDE project URI

• PRIDE
  1. PXD013506
     1. Label: PRIDE project
     2. Name: Proteomic comparison of Prochlorococcus marinus ecotypes MED4, MIT9319 and SS120.