PXD013501

PXD013501 is an original dataset announced via ProteomeXchange.

Title	Human HEK293T Label-free proteomics
Description	DDX21 is an RNA helicase protein with a diverse set of biological functions. Abnormal levels of DDX21 protein has been observed in colorectal and breast cancers as well as in schizophrenic patients, making it a potential therapeutic target. DDX21 can bind and unwind guanine-rich four-stranded structures of RNA referred to as guanine-quadruplexes (rG4s) and affect the translation of a reporter construct with an rG4 in its 5’ UTR. However, no biological rG4 targets of DDX1 are currently known. In our current study, we use shotgun proteomics to compare global protein expression of cells with wild type DDX21 and mutant DDX21 with impaired rG4s binding capability to identify potential binding targets of DDX21.
HostingRepository	PRIDE
AnnounceDate	2019-11-07
AnnouncementXML	Submission_2019-11-07_00:43:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ramanaguru Siva Piragasam
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-15 02:21:55	ID requested
⏵ 1	2019-11-07 00:46:11	announced

McRae EKS, Dupas SJ, Booy EP, Piragasam RS, Fahlman RP, McKenna SA, An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21. RNA, 26(1):44-57(2020) [pubmed]

submitter keyword: Human, HEK293T, LC-MS/MS, QExactive

Sean McKenna
contact affiliation	Department of Chemistry, University of Manitoba, Canada
contact email	Sean.McKenna@umanitoba.ca
lab head
Ramanaguru Siva Piragasam
contact affiliation	University of Alberta
contact email	sivapira@ualberta.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD013501

PRIDE project URI

• PRIDE
  1. PXD013501
     1. Label: PRIDE project
     2. Name: Human HEK293T Label-free proteomics