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DataSet Summary

  • HostingRepository: jPOST
  • AnnounceDate: 2019-04-15
  • AnnouncementXML: Submission_2019-04-14_23:16:53.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Non peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Kouhei Nishino
  • Title: HeLa cell proteome: Different amounts of the tryptic peptide mixtures for LC-MS/MS
  • Description: The tryptic peptide mixture from HeLa cells was subjected to LC-MS/MS in triplicate each for 250 ng, 500 ng, 750 ng and 1000 ng peptides. LC-MS/MS was conducted using a Q Exactive mass spectrometer (Thermo Fisher Scientific) equipped with an Ultimate 3000 RSLCnano liquid chromatograph (Thermo Fisher Scientific) containing C18 capillary LC column (Nano HPLC capillary Column, 75 μm i.d., 150 mm length, 3 μm particle size, Nikkyo Technos, Tokyo, Japan). LC-MS/MS data were integrated on a Proteome Discoverer 2.2 software (Thermo Fisher Scientific), followed by sequence database search of the MS/MS data using Mascot software (Matrix Science). The search results showed identification of the total of 3649 protein groups, filtered at 1% false discovery rate (FDR) peptide threshold.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02019-04-14 23:16:50ID requested
12019-04-14 23:16:54announced

Publication List

  1. Dataset with its publication pending

Keyword List

  1. submitter keyword: HeLa cells, LFQ

Contact List

    Takao Kawakami
    • lab head:
    Kouhei Nishino
    • contact affiliation: Medical ProteoScope Co., Ltd.
    • dataset submitter:

Full Dataset Link List

  1. jPOST dataset URI
  2. Dataset FTP location

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