PXD013490

PXD013490 is an original dataset announced via ProteomeXchange.

Title	Inhibition of mPGES-1 or COX-2 Results in Different Proteomic and Lipidomic Profiles in A549 Lung Cancer Cells
Description	We compared the effects of mPGES-1 inhibitor Compound III (CIII) with the cyclooxygenase (COX)-2 inhibitor NS-398 on protein and lipid profiles in interleukin (IL)-1β induced A549 lung cancer cells using mass spectrometry. Inhibition of mPGES-1 decreased PGE2 production and increased PGF2α and thromboxane B2 (TXB2) formation, while inhibition of COX-2 decreased the production of all three prostanoids. Our proteomics results revealed that CIII downregulated multiple canonical pathways including eIF2, eIF4/P70S6K, and mTOR signaling, compared to NS-398 that activated these pathways.
HostingRepository	PRIDE
AnnounceDate	2019-05-20
AnnouncementXML	Submission_2019-06-26_00:26:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elena Ossipova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N6: N6-dimethyl-L-lysine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-12 07:38:09	ID requested
1	2019-05-20 00:16:47	announced
⏵ 2	2019-06-26 00:26:41	announced	Updated publication reference for PubMed record(s): 31231223.

Bergqvist F, Ossipova E, Idborg H, Raouf J, Checa A, Englund K, Englund P, Khoonsari PE, Kultima K, Wheelock CE, Larsson K, Korotkova M, Jakobsson PJ, Inhibition of mPGES-1 or COX-2 Results in Different Proteomic and Lipidomic Profiles in A549 Lung Cancer Cells. Front Pharmacol, 10():636(2019) [pubmed]

submitter keyword: cancer, mPGES-1, inflammation, A549, LC-MS/MS

Per-Johan Jakobsson
contact affiliation	Division of Rheumatology, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden
contact email	Per-Johan.Jakobsson@ki.se
lab head
Elena Ossipova
contact affiliation	Karolinska Institute
contact email	Elena.Ossipova@ki.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013490
     1. Label: PRIDE project
     2. Name: Inhibition of mPGES-1 or COX-2 Results in Different Proteomic and Lipidomic Profiles in A549 Lung Cancer Cells