PXD013486

PXD013486 is an original dataset announced via ProteomeXchange.

Title	SCX, 18O-labeling and charge reversal for C-terminomics
Description	The proteome wide, mass spectrometry based identification of protein C-termini is hampered by factors such as poor ionization efficiencies, low yields in labeling strategies or the need for enrichment procedures. We present a bottom-up proteomics workflow to identify protein C-termini utilizing a combination of strong cation exchange chromatography, on-solid phase charge-reversal derivatization and LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:55:07.499.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-12 05:53:31	ID requested
1	2019-06-20 02:54:49	announced
⏵ 2	2024-10-22 04:55:08	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.9b00264;

Kaleja P, Helbig AO, Tholey A, Combination of SCX Fractionation and Charge-Reversal Derivatization Facilitates the Identification of Nontryptic Peptides in C-Terminomics. J Proteome Res, 18(7):2954-2964(2019) [pubmed]

curator keyword: Biological

submitter keyword: LC-MS, Charge-reversal derivatization,C-terminomics

Andreas Tholey
contact affiliation	Systematische Proteomforschung & Bioanalytik, Institut für Experimentelle Medizin, Christian-Albrechts-Universität zu Kiel, Niemannsweg 11, 24105 Kiel, Germany
contact email	a.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD013486

PRIDE project URI

• PRIDE
  1. PXD013486
     1. Label: PRIDE project
     2. Name: SCX, 18O-labeling and charge reversal for C-terminomics