PXD013484

PXD013484 is an original dataset announced via ProteomeXchange.

Title	O-GalNAc–glycosylated nuclear proteins
Description	Biological functions of nuclear proteins are regulated by post-translational modifications (PTMs) that modulate gene expression and cellular physiology. However, the role of O-linked glycosylation (O-GalNAc) as a PTM of nuclear proteins in the human cell has not been previously reported. Here, we examined the initiation of O-GalNAc glycan biosynthesis, representing a novel PTM of nuclear proteins in the nucleus of human cells, with an emphasis on HeLa cells. Using affinity chromatography and MS analyses, we identified O-GalNAc glycosylated proteins in the nucleus and present solid evidence for O-GalNAc glycan synthesis in this organelle. The demonstration of O-GalNAc glycosylation of nuclear proteins in mammalian cells reported here has important implications for cell and chemical biology.
HostingRepository	PRIDE
AnnounceDate	2019-04-17
AnnouncementXML	Submission_2019-04-17_10:43:49.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013484
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	ROMINA CEJAS
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	residues isobaric at 113.084064 Da; Oxidation; Carbamidomethyl; complex glycosylation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-12 04:14:45	ID requested
⏵ 1	2019-04-17 10:43:50	announced

Cejas RB, Lorenz V, Garay YC, Irazoqui FJ, -acetylgalactosamine glycans in the human cell nucleus. J Biol Chem, 294(9):2997-3011(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: O-GalNAc, nuclear glysosylation, HeLa

FERNANDO J IRAZOQUI
contact affiliation	Centro de Investigaciones en Química Biológica de Córdoba, CIQUIBIC, CONICET, and Departamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, X5000HUA Córdoba, Argentina
contact email	irazoqui@fcq.unc.edu.ar
lab head
ROMINA CEJAS
contact affiliation	SCHOOL OF PHARMACY AND PHARM SCI
contact email	rominabcejas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD013484

PRIDE project URI

• PRIDE
  1. PXD013484
     1. Label: PRIDE project
     2. Name: O-GalNAc–glycosylated nuclear proteins