PXD013482

PXD013482 is an original dataset announced via ProteomeXchange.

Title	Changes in the turnover of the cellular proteome during metabolic reprogramming: A role for mtROS in proteostasis
Description	The role played by protein turnover in metabolic reprogramming is unknown. Herein, using a SILAC approach, we have studied the changes in the half-life of two hundred and sixty-six proteins of energy metabolism and of translation during the metabolic switch induced by the prolyl hydroxylases inhibitor dimethyloxalylglycine (DMOG). DMOG induces HIF-1α expression and triggers the activation of glycolysis and the concurrent inhibition of mitochondrial respiration in colon cancer cells. Changes in the activity of energy provision pathways correlated with increased turnover rates of glycolytic enzymes and the stabilization of mitochondrial proteins. Moreover, reprograming also stabilized the proteins of translation. The partial DMOG-mediated arrest of the synthesis of mitochondrial and translation proteins results from the inhibition of the mTORC1/p70SK/S6 signaling pathway. In contrast, DMOG stimulated the synthesis of glycolytic enzymes, emphasizing the opposite and differential regulation of the two pathways of energy provision. Addition of MitoQ, a mitochondrial reactive oxygen species (mtROS) scavenger, stabilized the turnover of cellular proteins similarly as when protein degradation is inhibited with leupeptin, a serine-protease inhibitor. Overall, the results show that the higher the activity of a pathway the lower is the half-life of the proteins involved and suggest a role for mtROS in cellular proteostasis.
HostingRepository	PRIDE
AnnounceDate	2019-07-15
AnnouncementXML	Submission_2019-07-15_01:57:02.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013482
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ana García Aguilar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	HNE; GG; Bacillosamine; Hex; Delta:H(4)C(2)O(-1)S(1); Propionamide; Deamidated; Methylpyrroline; Methylamine; azole; DTT_ST; Myristoyl; NO_SMX_SMCT; Gln->pyro-Glu; Label:13C(6)15N(2); Diethylphosphate; Guanidinyl; DAET; DeStreak; Dehydro; Trimethyl; Ammonia-loss; Nethylmaleimide+water; Oxidation; ISD_z+2_ion; Carboxy; O-pinacolylmethylphosphonate; 4-ONE; Ethanolamine; Quinone; Cation:Na; Nitrosyl; Hydroxytrimethyl; Sulfo; Deoxy; Pyro-carbamidomethyl; Methyl+Deamidated; Malonyl; HPG; Formyl; glucosone; Carbamyl; LG-anhydrolactam; Cation:Mg[II]; 3-deoxyglucosone; Sulfide; NDA; Amino; Ammonium; Dimethyl; Menadione; Delta:H(5)C(2); Hypusine; Dethiomethyl; Amidated; Diethyl; Thioacyl; Cys->Dha; Ethanolyl; Pro->pyro-Glu; Phospho; Propargylamine; Acetyl; Nmethylmaleimide+water; Nitro; Methylthio; Carboxymethyl; Carbamidomethyl; Cation:Ca[II]; Trioxidation; Glycerophospho; Glu->pyro-Glu; Hydroxymethyl; Delta:H(2)C(2); Cation:Li; Ethyl; GPIanchor; BHT; Cation:Fe[II]; Cyano; Isopropylphospho; Methyl; AEBS; Pyridylacetyl; Dihydroxyimidazolidine; Methylmalonylation; Pyridylethyl; Didehydro; Trp->Kynurenin; Arg->GluSA; SMA; Hydroxycinnamyl; Nmethylmaleimide; DimethylpyrroleAdduct; G-H1; Lys->Allysine; Amidine; ethylamino; Label:13C(6)15N(4); maleimide; O-Methylphosphate; Dehydrated; Biotin; Dioxidation; Ethyl+Deamidated; Carbofuran; Benzoyl; glycidamide
Instrument	TripleTOF 5600; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-12 03:14:30	ID requested
⏵ 1	2019-07-15 01:57:04	announced

Garc, í, a-Aguilar A, Mart, í, nez-Reyes I, Cuezva JM, Changes in the Turnover of the Cellular Proteome during Metabolic Reprogramming: A Role for mtROS in Proteostasis. J Proteome Res, 18(8):3142-3155(2019) [pubmed]

submitter keyword: ATP synthase, Glycolysis, mitochondrial ROS, mTORC1, prolyl hydroxylases, SILAC.

José Manuel Cuezva Marcos
contact affiliation	Centro de Biología Molecular "Severo Ochoa" Universidad Autónoma de Madrid, 28049 Madrid, SPAIN
contact email	jmcuezva@cbm.uam.es
lab head
Ana García Aguilar
contact affiliation	Universidad Autónoma de Madrid
contact email	ana.garcia@cbm.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD013482

PRIDE project URI

• PRIDE
  1. PXD013482
     1. Label: PRIDE project
     2. Name: Changes in the turnover of the cellular proteome during metabolic reprogramming: A role for mtROS in proteostasis