PXD013479

PXD013479 is an original dataset announced via ProteomeXchange.

Title	Seed proteome of pea lines with different responsivity to simultaneous inoculation with arbuscular mycorrhiza fungi and rhizobia
Description	Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms – rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these interactions depends on plant genotype. Recently, we have shown that after simultaneous inoculation with Rh and AM, the productivity gain of pea (Pisum sativum L) line K-8274, characterized with a high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358. However, the molecular mechanisms behind this effect are still uncharacterized. Therefore, here, we address the alterations in pea seed proteome, underlying the symbiosis-related productivity gain, and identify 111 proteins, differentially expressed in two lines. The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis and down-regulation of LEA proteins. In contrast, the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism. Thus, we assume that the EIBSM trait is due to by prolongation of seed filling that needs to be taken into account when designing production of new pulse crops.
HostingRepository	PRIDE
AnnounceDate	2019-05-03
AnnouncementXML	Submission_2019-05-03_14:16:31.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013479
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tatiana Mamontova
SpeciesList	scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-12 02:31:55	ID requested
⏵ 1	2019-05-03 14:16:33	announced

Mamontova T, Afonin AM, Ihling C, Soboleva A, Lukasheva E, Sulima AS, Shtark OY, Akhtemova GA, Povydysh MN, Sinz A, Frolov A, Zhukov VA, Tikhonovich IA, L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi. Molecules, 24(8):(2019) [pubmed]

submitter keyword: arbuscular mycorrhiza

beneficial soil microorganisms

combined inoculation

inoculation efficiency

LC-MS

pea (Pisum sativum L.)

proteomics

rhizobial symbiosis

seed proteome

Andrej Frolov
contact affiliation	Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
contact email	afrolov@ipb-halle.de
lab head
Tatiana Mamontova
contact affiliation	Saint Petersburg State University
contact email	mamontova-bio@mail.ru
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD013479

PRIDE project URI

• PRIDE
  1. PXD013479
     1. Label: PRIDE project
     2. Name: Seed proteome of pea lines with different responsivity to simultaneous inoculation with arbuscular mycorrhiza fungi and rhizobia