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PXD013470

PXD013470 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCryoEM Structures of Arabidopsis DDR Complexes Involved in RNA-directed DNA Methylation
DescriptionChemical cross-linking coupled to mass spectrometry was used to study the A. thaliana DDR complex consisting of DMS3, RMD1 and a peptide from the interaction region of DRD1. Cross-linking was performed using different cross-linking chemistries: disuccinimidyl suberate (DSS) and a combination of adipic acid dihydrazide (ADH) and the coupling reagent, DMTMM.
HostingRepositoryPRIDE
AnnounceDate2019-10-10
AnnouncementXMLSubmission_2019-10-10_08:44:42.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlexander Leitner
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-12 01:06:58ID requested
12019-07-15 00:59:32announced
22019-10-10 08:44:44announced2019-10-10: Updated project metadata.
Publication List
Wongpalee SP, Liu S, Gallego-Bartolom, é J, Leitner A, Aebersold R, Liu W, Yen L, Nohales MA, Kuo PH, Vashisht AA, Wohlschlegel JA, Feng S, Kay SA, Zhou ZH, Jacobsen SE, CryoEM structures of Arabidopsis DDR complexes involved in RNA-directed DNA methylation. Nat Commun, 10(1):3916(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Chemical cross-linking
DSS
ADH
DMTMM
structural proteomics
DDR complex
Contact List
Steve E. Jacobsen
contact affiliationDepartment of Molecular, Cellular and Developmental Biology, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA
contact emailjacobsen@ucla.edu
lab head
Alexander Leitner
contact affiliationETH Zurich, Institute of Molecular Systems Biology
contact emailleitner@imsb.biol.ethz.ch
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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