PXD013470

PXD013470 is an original dataset announced via ProteomeXchange.

Title	CryoEM Structures of Arabidopsis DDR Complexes Involved in RNA-directed DNA Methylation
Description	Chemical cross-linking coupled to mass spectrometry was used to study the A. thaliana DDR complex consisting of DMS3, RMD1 and a peptide from the interaction region of DRD1. Cross-linking was performed using different cross-linking chemistries: disuccinimidyl suberate (DSS) and a combination of adipic acid dihydrazide (ADH) and the coupling reagent, DMTMM.
HostingRepository	PRIDE
AnnounceDate	2019-10-10
AnnouncementXML	Submission_2019-10-10_08:44:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Leitner
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-12 01:06:58	ID requested
1	2019-07-15 00:59:32	announced
⏵ 2	2019-10-10 08:44:44	announced	2019-10-10: Updated project metadata.

Wongpalee SP, Liu S, Gallego-Bartolom, é J, Leitner A, Aebersold R, Liu W, Yen L, Nohales MA, Kuo PH, Vashisht AA, Wohlschlegel JA, Feng S, Kay SA, Zhou ZH, Jacobsen SE, CryoEM structures of Arabidopsis DDR complexes involved in RNA-directed DNA methylation. Nat Commun, 10(1):3916(2019) [pubmed]

curator keyword: Biological

submitter keyword: Chemical cross-linking

DSS

ADH

DMTMM

structural proteomics

DDR complex

Steve E. Jacobsen
contact affiliation	Department of Molecular, Cellular and Developmental Biology, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA
contact email	jacobsen@ucla.edu
lab head
Alexander Leitner
contact affiliation	ETH Zurich, Institute of Molecular Systems Biology
contact email	leitner@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD013470

PRIDE project URI

• PRIDE
  1. PXD013470
     1. Label: PRIDE project
     2. Name: CryoEM Structures of Arabidopsis DDR Complexes Involved in RNA-directed DNA Methylation