PXD013470 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CryoEM Structures of Arabidopsis DDR Complexes Involved in RNA-directed DNA Methylation |
Description | Chemical cross-linking coupled to mass spectrometry was used to study the A. thaliana DDR complex consisting of DMS3, RMD1 and a peptide from the interaction region of DRD1. Cross-linking was performed using different cross-linking chemistries: disuccinimidyl suberate (DSS) and a combination of adipic acid dihydrazide (ADH) and the coupling reagent, DMTMM. |
HostingRepository | PRIDE |
AnnounceDate | 2019-10-10 |
AnnouncementXML | Submission_2019-10-10_08:44:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexander Leitner |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-12 01:06:58 | ID requested | |
1 | 2019-07-15 00:59:32 | announced | |
⏵ 2 | 2019-10-10 08:44:44 | announced | 2019-10-10: Updated project metadata. |
Publication List
Wongpalee SP, Liu S, Gallego-Bartolom, é J, Leitner A, Aebersold R, Liu W, Yen L, Nohales MA, Kuo PH, Vashisht AA, Wohlschlegel JA, Feng S, Kay SA, Zhou ZH, Jacobsen SE, CryoEM structures of Arabidopsis DDR complexes involved in RNA-directed DNA methylation. Nat Commun, 10(1):3916(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Chemical cross-linking |
DSS |
ADH |
DMTMM |
structural proteomics |
DDR complex |
Contact List
Steve E. Jacobsen |
contact affiliation | Department of Molecular, Cellular and Developmental Biology, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA |
contact email | jacobsen@ucla.edu |
lab head | |
Alexander Leitner |
contact affiliation | ETH Zurich, Institute of Molecular Systems Biology |
contact email | leitner@imsb.biol.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013470
- Label: PRIDE project
- Name: CryoEM Structures of Arabidopsis DDR Complexes Involved in RNA-directed DNA Methylation