PXD013468

PXD013468 is an original dataset announced via ProteomeXchange.

Title	The interactome of the clathrin adaptor AP2 is modulated by the phosphorylation status of its µ2 subunit.
Description	The clathrin adaptor AP2 is phosphorylated at a single site (Thr156) of its µ2 subunit. To gain insight how phosphorylation influences the interactome of AP2, we used human RPE cells which that were treated with LP-935509, an inhibitor of the adaptor-associated kinase AAK1. Kinase inhibition and loss of µ2 phosphorylation was verified with phospho-specific antibodies in westernblots of total cell extracts. We then used SILAC labelled cells and immune-isolated AP-2 and subjected bound proteins to mass spectrometry to identify all bound proteins and to determine phosphorylation-dependent changes in the interactome.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-11_17:31:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Hoening
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-12 00:36:48	ID requested
⏵ 1	2019-11-11 17:31:58	announced

Wrobel AG, Kadlecova Z, Kamenicky J, Yang JC, Herrmann T, Kelly BT, McCoy AJ, Evans PR, Martin S, M, ü, ller S, Salomon S, Sroubek F, Neuhaus D, H, ö, ning S, Owen DJ, Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation. Dev Cell, 50(4):494-508.e11(2019) [pubmed]

curator keyword: Biological

submitter keyword: Endocytosis, clathrin, receptor, Necap, AAK1

Stefan Höning
contact affiliation	Institute of Biochemistry Medical Faculty University of Cologne Germany
contact email	shoening@uni-koeln.de
lab head
Stefan Hoening
contact affiliation	Institute for Biochemistry, Medical Faculty, University of Cologne
contact email	shoening@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD013468

PRIDE project URI

• PRIDE
  1. PXD013468
     1. Label: PRIDE project
     2. Name: The interactome of the clathrin adaptor AP2 is modulated by the phosphorylation status of its µ2 subunit.