PXD013466

PXD013466 is an original dataset announced via ProteomeXchange.

Title	Assessing the effect of leptin central administration on plasma proteins in insulin deficient mice.
Description	Leptin monotherapy (i.e. without the use of administered insulin and/or any other molecule) corrects ID-induced metabolic aberrancies and promotes survival of insulin deficient rodents. These results generated great interest in the possibility of treating insulin deficient patients with leptin and/or molecule(s) underlying its beneficial effects. Hence, with the goal of identifying circulating molecule(s) underlying the advantageous effect of leptin we performed quantitative proteomic analysis of plasma and identified S100A9 as a putative peripheral mediator of leptin action. Here, to identify circulating molecule(s) underlying the advantageous effect of leptin we compared the results obtained by quantitative proteomic analysis of plasma between 2 groups of mice: streptozotocin (STZ)-treated mice that underwent intracerebroventricular (icv) leptin treatment for 12 days (STZ-Leptin) and ii) STZ-treated mice that underwent icv leptin treatment for 10 days and were withdrawn from leptin treatment for the following two days (STZ-Leptin-STOP). STZ treatment led to a massive loss of pancreatic insulin-producing β-cells, diminished pancreatic Proinsulin mRNA level, and caused severe insulinopenia, and hyperglycemia. icv leptin administration normalized hyperglycemia. However, two days after leptin delivery was halted hyperglycemia reappeared. We hypothesized that change in plasmatic protein(s) content could underlie re-emergence of hyperglycemia following decrease of leptin action.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:56:14.561.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giorgio Ramadori
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-12 00:04:02	ID requested
1	2019-07-12 03:13:10	announced
2	2019-08-12 01:36:20	announced	Updated publication reference for PubMed record(s): 31391467.
⏵ 3	2024-10-22 04:56:16	announced	2024-10-22: Updated project metadata.

Ramadori G, Ljubicic S, Ricci S, Mikropoulou D, Brenachot X, Veyrat-Durebex C, Aras E, Ioris RM, Altirriba J, Malle E, Foell D, Vogl T, Coppari R, S100A9 extends lifespan in insulin deficiency. Nat Commun, 10(1):3545(2019) [pubmed]

10.1038/s41467-019-11498-x;

curator keyword: Biological

submitter keyword: Glycemia, Brain,Leptin, Insulin deficiency, Plasma

Giorgio Ramadori
contact affiliation	Department of Cell Physiology and Metabolism, Faculty of medicine, University of Geneva
contact email	giogio.ramadori@unige.ch
lab head
Giorgio Ramadori
contact affiliation	University of Geneva
contact email	giorgio.ramadori@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD013466

PRIDE project URI

• PRIDE
  1. PXD013466
     1. Label: PRIDE project
     2. Name: Assessing the effect of leptin central administration on plasma proteins in insulin deficient mice.