PXD013458

PXD013458 is an original dataset announced via ProteomeXchange.

Title	Methylated trypsin
Description	HCP analysis of the various trypsins was performed using the X! Tandem search engine (Alanine) (http://www.thegpm.org). All data was searched against the downloaded UniProt Sus scrofa proteome (40.706 sequences, March 10, 2019) and the most recent cRAP list (http://www.thegpm.org). Data were search as: enzyme: semi-tryptic, parent ion error: 10 ppm, fragment error: 0.1 Da, max e-value: 0.01, fixed modifications: carbamidomethylation (C), partial modifications: oxidation (M), methylation (K), dimethylation (K). Each triplicate set of result files were then analyzed through PeptideShaker 1.16.38 [26] and reported at 1% FDR.
HostingRepository	PRIDE
AnnounceDate	2019-06-06
AnnouncementXML	Submission_2019-07-09_04:50:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sigurd Josef Frederiksen
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	monomethylated residue; carbamoylated residue; monohydroxylated residue; dimethylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-11 04:09:18	ID requested
1	2019-06-06 03:25:16	announced
⏵ 2	2019-07-09 04:50:50	announced	Updated publication reference for PubMed record(s): 31246970.

Heissel S, Frederiksen SJ, Bunkenborg J, H, ø, jrup P, Enhanced trypsin on a budget: Stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications. PLoS One, 14(6):e0218374(2019) [pubmed]

submitter keyword: trypsin, methylation, HCP

Peter Højrup
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
contact email	php@bmb.sdu.dk
lab head
Sigurd Josef Frederiksen
contact affiliation	University of Southern Denmark
contact email	sjfred13@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD013458

PRIDE project URI

• PRIDE
  1. PXD013458
     1. Label: PRIDE project
     2. Name: Methylated trypsin