PXD013446

PXD013446 is an original dataset announced via ProteomeXchange.

Title	Novel functions of the ubiquitin-independent proteasome system in regulating Xenopus germline development
Description	In most species, early germline development occurs in the absence of transcription with germline determinants subject to complex translational and post-translational regulations. Here, we report for the first time that early germline development is influenced by dynamic regulation of the proteasome system, previously thought to be ubiquitously expressed and to serve ‘housekeeping’ roles in controlling protein homeostasis. We show that proteasomes are present in a gradient with highest levels in the animal hemisphere but extending into the vegetal hemisphere of Xenopus oocytes. This distribution changes dramatically during the oocyte-to-embryo transition, with proteasomes becoming enriched in and restricted to the animal hemisphere and therefore separated from vegetally localized germline determinants. We identify Dead-end1 (Dnd1), a master regulator of vertebrate germline development, as a novel substrate of the ubiquitin-independent proteasomes. In the oocyte, ubiquitin-independent proteasomal degradation acts together with translational repression to prevent premature accumulation of Dnd1 protein. In the embryo, artificially increasing ubiquitin-independent proteasomal degradation in the vegetal pole interferes with germline development. Our work thus reveals novel inhibitory functions and spatial regulation of the ubiquitin-independent proteasome during vertebrate germline development.
HostingRepository	PRIDE
AnnounceDate	2019-04-11
AnnouncementXML	Submission_2019-05-27_01:08:24.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013446
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zuo-Fei Yuan
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	phosphorylated residue; formylated residue; acetylated residue; propanoylated residue; butanoylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-10 06:08:29	ID requested
1	2019-04-10 12:09:39	announced
2	2019-04-11 00:31:19	announced	Updated project metadata.
⏵ 3	2019-05-27 01:08:25	announced	Updated project metadata.

Hwang H, Jin Z, Krishnamurthy VV, Saha A, Klein PS, Garcia B, Mei W, King ML, Zhang K, Yang J, germline development. Development, 146(8):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Ubiquitin-independent proteasome, Dnd1, Germline development, Oocyte-to-embryo transition, Xenopus

Benjamin A. Garcia
contact affiliation	Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine
contact email	bgarci@pennmedicine.upenn.edu
lab head
Zuo-Fei Yuan
contact affiliation	UPenn
contact email	zuoyuan@mail.med.upenn.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013446
     1. Label: PRIDE project
     2. Name: Novel functions of the ubiquitin-independent proteasome system in regulating Xenopus germline development