PXD013443

PXD013443 is an original dataset announced via ProteomeXchange.

Title	Polyamines and eIF5A hypusination modulate mitochondrial respiration and macrophage activation
Description	How cells adapt metabolism to meet demands is an active area of interest across biology. Amongst a broad range of functions, the polyamine spermidine is needed to hypusinate the translation factor eukaryotic initiation factor 5A (eIF5A). We show here that hypusinated eIF5A (eIF5AH) promotes the efficient expression of a subset of mitochondrial proteins involved in the TCA cycle and oxidative phosphorylation (OXPHOS). Several of these proteins have mitochondrial targeting sequences (MTS) that in part, confer an increased dependency on eIF5AH. In macrophages, metabolic switching between OXPHOS and glycolysis supports divergent functional fates stimulated by activation signals.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:55:46.339.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yaarub Musa
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-10 04:55:13	ID requested
1	2019-05-24 00:24:59	announced
2	2019-05-31 02:35:42	announced	Updated publication reference for PubMed record(s): 31130465.
⏵ 3	2024-10-22 04:55:47	announced	2024-10-22: Updated project metadata.

10.1016/j.cmet.2019.05.003;

Puleston DJ, Buck MD, Klein Geltink RI, Kyle RL, Caputa G, O'Sullivan D, Cameron AM, Castoldi A, Musa Y, Kabat AM, Zhang Y, Flachsmann LJ, Field CS, Patterson AE, Scherer S, Alfei F, Baixauli F, Austin SK, Kelly B, Matsushita M, Curtis JD, Grzes KM, Villa M, Corrado M, Sanin DE, Qiu J, P, ä, llman N, Paz K, Maccari ME, Blazar BR, Mittler G, Buescher JM, Zehn D, Rospert S, Pearce EJ, Balabanov S, Pearce EL, Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation. Cell Metab, 30(2):352-363.e8(2019) [pubmed]

curator keyword: Biological

submitter keyword: proteomics, macrophage, polyamine,eIF5A

Dr. Gerhard Mittler
contact affiliation	Dr. Gerhard Mittler Group Leader Proteomics Unit MPI Immunobiology and Epigenetics Stuebeweg 51 D-79108 Freiburg, Germany Phone: +49 (0)761 5108 712
contact email	mittler@ie-freiburg.mpg.de
lab head
Yaarub Musa
contact affiliation	Max Planck Institute for Immunobiology and Epigenetics
contact email	musa@ie-freiburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD013443

PRIDE project URI

• PRIDE
  1. PXD013443
     1. Label: PRIDE project
     2. Name: Polyamines and eIF5A hypusination modulate mitochondrial respiration and macrophage activation