PXD013440

PXD013440 is an original dataset announced via ProteomeXchange.

Title	Characterisation of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite
Description	Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host. Here we have identified and characterised a sigma class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b). O. ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms. A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion. Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)/MS following a coupled-enzyme assay. This is only the second reported and characterised glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:05:08.772.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stuart Armstrong
SpeciesList	scientific name: Onchocerca ochengi (Filarial nematode worm); NCBI TaxID: 42157;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q-Tof micro

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-10 03:27:04	ID requested
1	2020-05-26 13:55:14	announced
⏵ 2	2024-10-22 05:05:14	announced	2024-10-22: Updated project metadata.

10.1017/s0031182019000763;

Rose C, Praulins G, Armstrong SD, Casas-Sanchez A, Davis J, Molyneux G, Yunta C, Stead Z, Prescott M, Perally S, Rutter A, Makepeace BL, La Course EJ, Acosta-Serrano A, , closest relative of the human river blindness parasite. Parasitology, 146(14):1773-1784(2019) [pubmed]

submitter keyword: glycosylation, Onchocerca, prostaglandin synthase, detoxification, glycans,Immune modulation, GSTs

James LaCourse
contact affiliation	Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool UK
contact email	James.LaCourse@lstmed.ac.uk
lab head
Stuart Armstrong
contact affiliation	Infection Biology
contact email	sarmstro@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013440
     1. Label: PRIDE project
     2. Name: Characterisation of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite