⮝ Full datasets listing

PXD013440

PXD013440 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterisation of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite
DescriptionFilarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host. Here we have identified and characterised a sigma class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b). O. ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms. A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion. Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)/MS following a coupled-enzyme assay. This is only the second reported and characterised glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:05:08.772.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStuart Armstrong
SpeciesList scientific name: Onchocerca ochengi (Filarial nematode worm); NCBI TaxID: 42157;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ-Tof micro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-10 03:27:04ID requested
12020-05-26 13:55:14announced
22024-10-22 05:05:14announced2024-10-22: Updated project metadata.
Publication List
10.1017/s0031182019000763;
Rose C, Praulins G, Armstrong SD, Casas-Sanchez A, Davis J, Molyneux G, Yunta C, Stead Z, Prescott M, Perally S, Rutter A, Makepeace BL, La Course EJ, Acosta-Serrano A, , closest relative of the human river blindness parasite. Parasitology, 146(14):1773-1784(2019) [pubmed]
Keyword List
submitter keyword: glycosylation, Onchocerca, prostaglandin synthase, detoxification, glycans,Immune modulation, GSTs
Contact List
James LaCourse
contact affiliationTropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool UK
contact emailJames.LaCourse@lstmed.ac.uk
lab head
Stuart Armstrong
contact affiliationInfection Biology
contact emailsarmstro@liv.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD013440
PRIDE project URI
Repository Record List
[ + ]