PXD013440 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterisation of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite |
Description | Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host. Here we have identified and characterised a sigma class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b). O. ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms. A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion. Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)/MS following a coupled-enzyme assay. This is only the second reported and characterised glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:05:08.772.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stuart Armstrong |
SpeciesList | scientific name: Onchocerca ochengi (Filarial nematode worm); NCBI TaxID: 42157; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q-Tof micro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-10 03:27:04 | ID requested | |
1 | 2020-05-26 13:55:14 | announced | |
⏵ 2 | 2024-10-22 05:05:14 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1017/s0031182019000763; |
Rose C, Praulins G, Armstrong SD, Casas-Sanchez A, Davis J, Molyneux G, Yunta C, Stead Z, Prescott M, Perally S, Rutter A, Makepeace BL, La Course EJ, Acosta-Serrano A, , closest relative of the human river blindness parasite. Parasitology, 146(14):1773-1784(2019) [pubmed] |
Keyword List
submitter keyword: glycosylation, Onchocerca, prostaglandin synthase, detoxification, glycans,Immune modulation, GSTs |
Contact List
James LaCourse |
contact affiliation | Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool UK |
contact email | James.LaCourse@lstmed.ac.uk |
lab head | |
Stuart Armstrong |
contact affiliation | Infection Biology |
contact email | sarmstro@liv.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013440
- Label: PRIDE project
- Name: Characterisation of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite