PXD013435 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RST1 and RIPR connect the cytosolic RNA exosome to the SKI complex in Arabidopsis |
Description | The RNA exosome is a key 3’-5’ exoribonuclease with evolutionary conserved structure and roles. Its cytosolic functions require the co-factors SKI7 and the Ski complex. Here we demonstrate by co-purification experiments that the ARM repeat protein RESURRECTION1 (RST1) and RST1 INTERACTING PROTEIN (RIPR) connect the cytosolic Arabidopsis RNA exosome to the Ski complex. rst1 and ripr mutants accumulate small RNAs many of which are quality control siRNAs (rqc-siRNAs) produced by the postranscriptional gene silencing (PTGS) machinery when mRNA degradation is compromised. Indeed, quasi identical small RNA populations are observed in mutants lacking the RRP45B/CER7 subunit of the core exosome. This biochemical and genetic evidence supports a physical and functional link between RST1, RIPR and the RNA exosome. Our data reveal the existence of additional cytosolic exosome co-factors besides the known SKI subunits. Interestingly, RST1 is not restricted to plants, as homologues with a similar domain architecture exist in animals, including humans. |
HostingRepository | PRIDE |
AnnounceDate | 2019-10-10 |
AnnouncementXML | Submission_2019-10-10_08:49:32.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lauriane Kuhn |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-10 03:23:58 | ID requested | |
1 | 2019-07-17 07:06:43 | announced | |
⏵ 2 | 2019-10-10 08:49:34 | announced | 2019-10-10: Updated project metadata. |
Publication List
Lange H, Ndecky SYA, Gomez-Diaz C, Pflieger D, Butel N, Zumsteg J, Kuhn L, Piermaria C, Chicher J, Christie M, Karaaslan ES, Lang PLM, Weigel D, Vaucheret H, Hammann P, Gagliardi D, RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis. Nat Commun, 10(1):3871(2019) [pubmed] |
Keyword List
submitter keyword: RST1, SKI complex, Exosome complex, RNA exosome, Exo9, Ski7 |
Contact List
Dominique Gagliardi |
contact affiliation | Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, UPR 2357, Université de Strasbourg, Strasbourg, France. |
contact email | dominique.gagliardi@ibmp-cnrs.unistra.fr |
lab head | |
Lauriane Kuhn |
contact affiliation | CNRS, IBMC, FRC1589 |
contact email | l.kuhn@ibmc-cnrs.unistra.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013435
- Label: PRIDE project
- Name: RST1 and RIPR connect the cytosolic RNA exosome to the SKI complex in Arabidopsis