PXD013432

PXD013432 is an original dataset announced via ProteomeXchange.

Title	Evolutionary compaction and adaptation visualized by the structure of the dormant microsporidian ribosome
Description	Microsporidia are eukaryotic parasites that infect essentially all animal species, including many of agricultural importance, and are significant opportunistic parasites of humans. They are characterized by having a specialized infection apparatus, an obligate intracellular lifestyle, rudimentary mitochondria and the smallest eukaryotic genomes. Extreme genome compaction has led to minimal gene sizes affecting even conserved ancient complexes such as the ribosome. Here we present the high-resolution cryo-EM structure of the microsporidian ribosome which illustrates how genome compaction has resulted in the smallest eukaryotic cytoplasmic ribosome. Selection pressure has led to the loss of two ribosomal proteins and removal of essentially all eukaryote-specific rRNA expansion segments reducing the ribosomal RNA to a functionally conserved core. The structure also highlights how one microsporidia-specific and several repurposed existing ribosomal proteins compensate for the extensive reduction of the rRNA. The microsporidian ribosome is kept in an inactive state by two previously uncharacterized dormancy factors that specifically target the functionally important E-site, P-site and polypeptide exit tunnel. This study visualizes the distinct effects of evolutionary pressure on RNA and protein coding genes, provides a new mechanism for ribosome inhibition and can serve as a structural basis for the development of small molecule inhibitors against microsporidian parasites.
HostingRepository	PRIDE
AnnounceDate	2019-07-02
AnnouncementXML	Submission_2019-07-29_01:01:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	henrik molina
SpeciesList	scientific name: Vairimorpha necatrix; NCBI TaxID: 6039;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-09 08:18:49	ID requested
1	2019-07-02 03:17:29	announced
⏵ 2	2019-07-29 01:01:51	announced	Updated publication reference for PubMed record(s): 31332387.

Barandun J, Hunziker M, Vossbrinck CR, Klinge S, Evolutionary compaction and adaptation visualized by the structure of the dormant microsporidian ribosome. Nat Microbiol, 4(11):1798-1804(2019) [pubmed]

submitter keyword: Microsporidia, ribosome, cryo-EM, mass spectrometry, Vairimorpha necatrix

Sebastian Klinge
contact affiliation	The Rockefeller University
contact email	klinge@mail.rockefeller.edu
lab head
henrik molina
contact affiliation	THE ROCKEFELLER UNIVERSITY
contact email	henrik.molina@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD013432

PRIDE project URI

• PRIDE
  1. PXD013432
     1. Label: PRIDE project
     2. Name: Evolutionary compaction and adaptation visualized by the structure of the dormant microsporidian ribosome