PXD013429

PXD013429 is an original dataset announced via ProteomeXchange.

Title	Human urinary extracellular vesicles protein LC-MS/MS
Description	Urinary extracellular vesicles (uEV) are a precious new source of biomarkers reflecting the physiological state of the upstream parent cells and organ systems. However, urinary Tamm-Horsfall Protein (THP; uromodulin) interferes heavily with all vesicle isolation attempts, precipitates with normal urinary proteins thus representing an unwanted “contaminant” in urinary assays. Here we developed a simple method to manage THP efficiently. Accordingly, uEV were isolated by hydrostatic filtration dialysis (HFD) and treated with a solution of urea to release most uEV. Presence of uEV was then confirmed by transmission electron microscopy, Western blot and proteomic profiling in MS, all showing that urea treatment did not affect integrity of uEV.
HostingRepository	PRIDE
AnnounceDate	2019-09-30
AnnouncementXML	Submission_2019-09-30_01:53:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaomeng Xu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-09 07:19:32	ID requested
⏵ 1	2019-09-30 01:53:26	announced

Xu X, Barreiro K, Musante L, Kretz O, Lin H, Zou H, Huber TB, Holthofer H, Management of Tamm-Horsfall Protein for Reliable Urinary Analytics. Proteomics Clin Appl, 13(6):e1900018(2019) [pubmed]

submitter keyword: Urinary Extracellular Vesicle, Tamm-Horsfall Protein, Urea, Mass Spectrometry, Biomarker

Harry Holthofer
contact affiliation	Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Finland
contact email	harry.holthoffer@helsinki.fi
lab head
Xiaomeng Xu
contact affiliation	The 3rd Affiliated Hospital Of Southern Medical University
contact email	xuxiaomeng@foxmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD013429

PRIDE project URI

• PRIDE
  1. PXD013429
     1. Label: PRIDE project
     2. Name: Human urinary extracellular vesicles protein LC-MS/MS