PXD013411

PXD013411 is an original dataset announced via ProteomeXchange.

Title	The signal transduction protein PII controls ammonium, nitrate and urea uptake in cyanobacteria
Description	PII signal transduction proteins are widely spread among all domains of life where they regulate a multitude of carbon and nitrogen metabolism related processes. Non-diazotrophic cyanobacteria can utilize a high variety of organic and inorganic nitrogen sources. In recent years, several physiological studies indicated an involvement of the cyanobacterial PII protein in regulation of ammonium, nitrate/nitrite and cyanate uptake. However, direct interaction of PII has not been demonstrated so far. In this study, we used biochemical, molecular genetic and physiological approaches to demonstrate that PII regulates all relevant nitrogen uptake systems in Synechocystis sp. strain PCC 6803: PII controls ammonium uptake by interacting with the Amt1 ammonium permease, probably similar to the known regulation of E. coli ammonium permease AmtB by the PII homologue GlnK. We could further clarify that PII mediates the ammonium- and dark-induced inhibition of nitrate uptake by interacting with the NrtC and NrtD subunits of the nitrate/nitrite transporter NrtABCD. We further identified the ABC-type urea transporter UrtABCDE as novel PII target. PII interacts with the UrtE subunit without involving the standard interaction surface of PII interactions. The deregulation of urea uptake in a PII deletion mutant causes ammonium excretion when urea is provided as nitrogen source. Furthermore, the urea hydrolyzing urease enzyme complex appears to be coupled to urea uptake. Overall, this study underlines the great importance of the PII signal transduction protein in the regulation of nitrogen utilization in cyanobacteria.
HostingRepository	PRIDE
AnnounceDate	2019-07-10
AnnouncementXML	Submission_2019-07-18_00:46:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philipp Spaet
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-09 01:17:50	ID requested
1	2019-07-10 00:24:21	announced
⏵ 2	2019-07-18 00:46:45	announced	Updated publication reference for PubMed record(s): 31293555.

Watzer B, Sp, ä, t P, Neumann N, Koch M, Sobotka R, Macek B, Hennrich O, Forchhammer K, Controls Ammonium, Nitrate and Urea Uptake in Cyanobacteria. Front Microbiol, 10():1428(2019) [pubmed]

curator keyword: Biological

submitter keyword: PII signaling protein, GlnB, Cyanobacteria, Nitrogen regulation, Nitrate uptake, Ammonium uptake, Urea uptake, ABC transporters

Prof. Dr. Karl Forchhammer
contact affiliation	Department of Organismic Interactions, University of Tübingen
contact email	karl.forchhammer@uni-tuebingen.de
lab head
Philipp Spaet
contact affiliation	University Tuebingen Department of Quantitative Proteomics
contact email	philipp.spaet@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013411
     1. Label: PRIDE project
     2. Name: The signal transduction protein PII controls ammonium, nitrate and urea uptake in cyanobacteria