PXD013403

PXD013403 is an original dataset announced via ProteomeXchange.

Title	Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery
Description	The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialised machinery that converts long single-stranded precursors into small RNAs of ~25-nucleotides in length. This process involves factors that operate in two different subcellular compartments: the nuage/Yb-bodies and mitochondria. How these two sites communicate to achieve accurate substrate selection and efficient processing remains unclear. Here, we investigate a previously uncharacterized piRNA biogenesis factor, Daedalus (Daed), that is located on the outer mitochondrial membrane. Daed is essential for Zucchini-mediated piRNA production and for the correct localisation of the indispensable piRNA biogenesis factor, Armitage (Armi). We find that Gasz and Daed interact with each other and likely provide a mitochondrial “anchoring platform” to ensure that Armi is held in place, proximal to Zucchini, during piRNA processing. Our data suggest that Armi initially identifies piRNA precursors in nuage/Yb-bodies in a manner that depends upon Piwi and then moves to mitochondria to present precursors to the mitochondrial biogenesis machinery. These results represent a significant step in understanding a critical aspect of transposon silencing, namely how RNAs are chosen to instruct the piRNA machinery in the nature of its silencing targets.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:02:34.045.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marzia Munafo
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	biotinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-08 07:22:22	ID requested
1	2019-04-18 06:10:58	announced
2	2019-05-31 02:37:13	announced	Updated publication reference for PubMed record(s): 31123065.
⏵ 3	2024-10-22 04:02:36	announced	2024-10-22: Updated project metadata.

Munaf, ò M, Manelli V, Falconio FA, Sawle A, Kneuss E, Eastwood EL, Seah JWE, Czech B, Hannon GJ, Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery. Genes Dev, 33(13-14):844-856(2019) [pubmed]

10.1101/gad.325662.119;

submitter keyword: pirna piwi drosophila

Gregory J Hannon
contact affiliation	Cancer Research UK Cambridge Institute University of Cambridge Li Ka Shing Centre Cambridge CB2 0RE United Kingdom
contact email	greg.hannon@cruk.cam.ac.uk
lab head
Marzia Munafo
contact affiliation	Cambridge University
contact email	marzia.munafo@cruk.cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013403
     1. Label: PRIDE project
     2. Name: Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery