PXD013402

PXD013402 is an original dataset announced via ProteomeXchange.

Title	SWATH-proteomics analysis of ibrutinib mechanism of action in neutropenia and acute myeloid leukemia initiating mutated granulocyte-colony stimulating factor receptor
Description	Neutrophil production and function are primarily determined by granulocyte colony stimulating factor receptor (G-CSFR). G-CSFRs associated mutations (mostly localized in the transmembrane and cytoplasmic domains of the receptor) have been reported with several distinct hematological abnormalities as well as malignancies, e.g. severe congenital neutropenia (SCN), acute myeloid leukemia (AML) and chronic neutrophilic leukemia (CNL). Ibrutinib, a small molecule Bruton’s tyrosine kinase (BTK) inhibitor, is FDA approved and clinically used against B-cell related leukemia. In our previous published work (Dwivedi et al., Leukemia.2019;33:75–87), we have shown ibrutinib’s efficacy in the mutated G-CSFRs based leukemia model systems (mouse and human). However, the signaling mechanism of ibrutinib’s efficacy is not explored yet. Here, we present a unique SWATH-based label free quantitative proteomics analysis of the normal and mutated G-CSFRs signaling post ibrutinib treatment, using 32D cell-line-based in vitro model system.
HostingRepository	PRIDE
AnnounceDate	2020-04-27
AnnouncementXML	Submission_2020-04-26_23:25:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Somchai Chutipongtanate
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-08 07:01:47	ID requested
⏵ 1	2020-04-26 23:25:19	announced

Dwivedi P, Chutipongtanate S, Muench DE, Azam M, Grimes HL, Greis KD, SWATH-Proteomics of Ibrutinib's Action in Myeloid Leukemia Initiating Mutated G-CSFR Signaling. Proteomics Clin Appl, 14(5):e1900144(2020) [pubmed]

submitter keyword: 32D, G-CSFR, BTK inhibitor, SWATH

Kenneth D. Greis
contact affiliation	UC Proteomics Laboratory, Department of Cancer Biology, University of Cincinnati, Cincinnati, Ohio 45267 USA
contact email	greiskd@ucmail.uc.edu
lab head
Somchai Chutipongtanate
contact affiliation	University of Cincinnati, Cincinnati, Ohio
contact email	schuti.rama@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD013402

PRIDE project URI

• PRIDE
  1. PXD013402
     1. Label: PRIDE project
     2. Name: SWATH-proteomics analysis of ibrutinib mechanism of action in neutropenia and acute myeloid leukemia initiating mutated granulocyte-colony stimulating factor receptor