PXD013398

PXD013398 is an original dataset announced via ProteomeXchange.

Title	TEFM regulates both transcription elongation and RNA processing in mitochondria
Description	Regulation of replication and expression of mitochondrial DNA (mtDNA) is essential for cellular energy conversion via oxidative phosphorylation. The mitochondrial transcription elongation factor (TEFM) has been proposed to regulate the switch between transcription termination for replication primer formation and processive, near-genome length transcription for mtDNA gene expression. Here, we report that Tefm is essential for mouse embryogenesis and that levels of promoter-distal mitochondrial transcripts are drastically reduced in conditional Tefm-knockout hearts. In contrast, the promoter-proximal transcripts are much increased in Tefm knockouts, but they mostly terminate before the region where the switch from transcription to replication occurs, and consequently de novo mtDNA replication is profoundly reduced. Unexpectedly, deep sequencing of RNA from Tefm knockouts revealed accumulation of unprocessed transcripts in addition to defective transcription elongation. Furthermore, a proximity labelling (BioID) assay showed that TEFM interacts with multiple RNA processing factors. Our data demonstrate that TEFM acts as a general transcription elongation factor, necessary for both gene transcription and replication primer formation, and loss of TEFM affects RNA processing in mammalian mitochondria.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:59:46.987.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-08 06:04:08	ID requested
1	2019-04-25 00:15:14	announced
2	2019-05-06 09:01:59	announced	Updated publication reference for PubMed record(s): 31036713.
⏵ 3	2024-10-22 03:59:47	announced	2024-10-22: Updated project metadata.

Jiang S, Koolmeister C, Misic J, Siira S, K, ü, hl I, Silva Ramos E, Miranda M, Jiang M, Posse V, Lytovchenko O, Atanassov I, Schober FA, Wibom R, Hultenby K, Milenkovic D, Gustafsson CM, Filipovska A, Larsson NG, TEFM regulates both transcription elongation and RNA processing in mitochondria. EMBO Rep, 20(6):(2019) [pubmed]

10.15252/embr.201948101;

submitter keyword: mtDNA replication, RNA processing, transcription elongation

Nils-Göran Larsson
contact affiliation	Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm 17177, Sweden Max Planck Institute Biology of Ageing - Karolinska Institutet Laboratory, Karolinska Institutet, Stockholm 17177, Sweden
contact email	nils-goran.larsson@ki.se
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	Ilian.Atanassov@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD013398

PRIDE project URI

• PRIDE
  1. PXD013398
     1. Label: PRIDE project
     2. Name: TEFM regulates both transcription elongation and RNA processing in mitochondria