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PXD013396

PXD013396 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChronic exposure to chewing tobacco induces metabolic reprogramming and cancer stem cell-like properties in esophageal epithelial cells
DescriptionTobacco in its smoke and smokeless form are major risk factors for ESCC (esophageal squamous cell carcinoma). However, molecular alterations associated with smokeless tobacco exposure are poorly understood. In the Indian subcontinent, tobacco is predominantly consumed in chewing form. An understanding of molecular alterations associated with chewing tobacco exposure is vital for identifying molecular markers and potential targets. We developed an in-vitro cellular model by exposing non-transformed esophageal epithelial cells to chewing tobacco over eight month period. Chronic exposure to chewing tobacco led to increase in cell proliferation, invasive ability and anchorage independent growth indicating cell transformation. Molecular alterations associated with chewing tobacco exposure were characterized by carrying out exome sequencing and quantitative proteomic profiling of parental cells and chewing tobacco exposed cells. Quantitative proteomic analysis revealed that established cancer stem cell markers are elevated in tobacco treated cells. Decreased expression of enzymes associated with the glycolytic pathway and increased expression of a large number of mitochondrially localized proteins involved in the electron transport chain as well as enzymes of TCA cycle were also identified. Electron micrographs revealed increase in number and size of mitochondria. Based on these observations, we hypothesise that chronic treatment of esophageal epithelial cells with tobacco leads to a cancer stem cell-like phenotype. These cells also show characteristic OXPHOS phenotype which can be potentially targeted as a therapeutic strategy.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_03:59:57.464.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHarsha Gowda
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-08 03:18:31ID requested
12019-11-11 15:25:57announced
22024-10-22 03:59:58announced2024-10-22: Updated project metadata.
Publication List
Datta KK, Patil S, Patel K, Babu N, Raja R, Nanjappa V, Mangalaparthi KK, Dhaka B, Rajagopalan P, Deolankar SC, Kannan R, Kumar P, Prasad TSK, Mathur PP, Kumari A, Manoharan M, Coral K, Murugan S, Sidransky D, Gupta R, Gupta R, Khanna-Gupta A, Chatterjee A, Gowda H, Chronic Exposure to Chewing Tobacco Induces Metabolic Reprogramming and Cancer Stem Cell-Like Properties in Esophageal Epithelial Cells. Cells, 8(9):(2019) [pubmed]
10.3390/cells8090949;
Keyword List
submitter keyword: cancer stem cells,Chewing tobacco, esophageal cancer, metabolic reprogramming
Contact List
Harsha Gowda
contact affiliationInstitute of Bioinformatics, International Tech Park, Bangalore 560066, India
contact emailharsha@ibioinformatics.org
lab head
Harsha Gowda
contact affiliationQIMR Berghofer Medical Research Institute
contact emailharsha@ibioinformatics.org
dataset submitter
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Dataset FTP location
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PRIDE project URI
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