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PXD013389

DataSet Summary

  • HostingRepository: PanoramaPublic
  • AnnounceDate: 2019-04-16
  • AnnouncementXML: Submission_2019-04-16_20:01:18.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Chris Petzold
  • Title: Biosynthesis and secretion of the microbial sulfated peptide RaxX and binding to the rice XA21 immune receptor
  • Description: The rice immune receptor XA21 is activated by the sulfated microbial peptide RaxX (required for activation of XA21-mediated immunity X) produced by Xanthomonas oryzae pv. oryzae (Xoo). Mutational studies and targeted proteomics revealed that RaxX is processed and secreted by the protease/transporter RaxB, whose function can be partially fulfilled by a noncognate peptidase-containing transporter B (PctB). RaxX is cleaved at a Gly-Gly motif, yielding a mature peptide that retains the necessary elements for RaxX function as an immunogen and host peptide hormone mimic. These results indicate that RaxX is a founding member of a previously unclassified and understudied group of tyrosine sulfated RiPPs (ribosomally synthesized, post-translationally modified peptides). We further demonstrate that sulfated RaxX directly binds XA21 with high affinity. This work reveals a complete, previously uncharacterized biological process: bacterial RiPP biosynthesis, secretion, binding to a eukaryotic receptor and triggering of a robust host immune response.
  • SpeciesList: scientific name: Xanthomonas oryzae pv. oryzae; NCBI TaxID: 64187;
  • ModificationList: Sulfo
  • Instrument: 6460 Triple Quadrupole LC/MS

Dataset History

VersionDatetimeStatusChangeLog Entry
02019-04-06 13:43:40ID requested
12019-04-16 20:01:20announced

Publication List

  1. Luu DD, Joe A, Chen Y, Parys K, Bahar O, Pruitt R, Chan LJG, Petzold CJ, Long K, Adamchak C, Stewart V, Belkhadir Y, Ronald PC, Biosynthesis and secretion of the microbial sulfated peptide RaxX and binding to the rice XA21 immune receptor. Proc Natl Acad Sci U S A, 116(17):8525-8534(2019) [pubmed]

Keyword List

  1. submitter keyword: RaxX, X. oryzae, XA21 immune receptor, Tyrosine sulfation, RiPPs

Contact List

    Chris Petzold
    • contact affiliation: Lawrence Berkeley National Laboratory
    • contact email: cjpetzold@lbl.gov
    • lab head:
    Chris Petzold
    • contact affiliation: Lawrence Berkeley National Laboratory
    • contact email: cjpetzold@lbl.gov
    • dataset submitter:

Full Dataset Link List

  1. Panorama Public dataset URI

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