PXD013373

PXD013373 is an original dataset announced via ProteomeXchange.

Title	Tpo1‐mediated spermine and spermidine export controls cell cycle delay and times antioxidant protein expression during the oxidative stress response
Description	Cells counteract oxidative stress by altering metabolism, cell cycle and gene expression. However, the mechanisms that coordinate these adaptations are only marginally understood. Here we provide evidence that timing of these responses in yeast requires export of the polyamines spermidine and spermine. We show that during hydrogen peroxide (H2O2) exposure, the polyamine transporter Tpo1 controls spermidine and spermine concentrations and mediates induction of antioxidant proteins, including Hsp70, Hsp90, Hsp104 and Sod1. Moreover, Tpo1 determines a cell cycle delay during adaptation to increased oxidant levels, and affects H2O2 tolerance. Thus, central components of the stress response are timed through Tpo1‐controlled polyamine export.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:58:48.163.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jakob Vowinckel
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-04 04:18:33	ID requested
1	2019-06-10 08:54:17	announced
2	2019-08-16 00:08:53	announced	Updated publication reference for PubMed record(s): 24136413, 31367038.
⏵ 3	2024-10-22 03:59:04	announced	2024-10-22: Updated project metadata.

Olin-Sandoval V, Yu JSL, Miller-Fleming L, Alam MT, Kamrad S, Correia-Melo C, Haas R, Segal J, Pe, ñ, a Navarro DA, Herrera-Dominguez L, M, é, ndez-Lucio O, Vowinckel J, M, ü, lleder M, Ralser M, Lysine harvesting is an antioxidant strategy and triggers underground polyamine metabolism. Nature, 572(7768):249-253(2019) [pubmed]

10.1038/embor.2013.165;

10.1038/s41586-019-1442-6;

Kr, ü, ger A, Vowinckel J, M, ü, lleder M, Grote P, Capuano F, Bluemlein K, Ralser M, Tpo1-mediated spermine and spermidine export controls cell cycle delay and times antioxidant protein expression during the oxidative stress response. EMBO Rep, 14(12):1113-9(2013) [pubmed]

curator keyword: Biological

submitter keyword: Yeast

Markus Ralser
contact affiliation	Einstein Professor of Biochemistry Head, Institute of Biochemistry, Charité University Medicine, Berlin
contact email	ralser359@gmail.com
lab head
Jakob Vowinckel
contact affiliation	Biognosys AG
contact email	jakob.vowinckel@biognosys.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD013373

PRIDE project URI

• PRIDE
  1. PXD013373
     1. Label: PRIDE project
     2. Name: Tpo1‐mediated spermine and spermidine export controls cell cycle delay and times antioxidant protein expression during the oxidative stress response