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PXD013359

PXD013359 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of several non pathogenic mutants from Botrytis cinerea.
DescriptionThe grey mould fungus Botrytis cinerea is a typical necrotrophic fungus that can infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to find new important genes involved in the fungal virulence, a mutant library was generated by random insertional mutagenesis, using Agrobacterium tumefaciens mediated transformation. Ten mutants exhibiting total loss of virulence towards different host plants were considered. Their molecular characterization revealed a single T-DNA insertion in unique and different loci. Using a proteomics approach, the secretome of four of these strains was compared to that of the parental strain and a common profile of reduced lytic enzymes was recorded. Significant variations in this profile, notably proteases and hemicellulases deficiencies, were observed and validated by biochemical tests. They were also a hallmark of the remaining six non pathogenic strains, suggesting the importance of these secreted proteins in the infectious process.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:30:09.989.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDupuy Jean-William
SpeciesList scientific name: Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea); NCBI TaxID: 40559;
ModificationListdeaminated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-03 06:57:09ID requested
12021-11-03 02:51:35announced
22024-10-22 05:30:12announced2024-10-22: Updated project metadata.
Publication List
10.3389/fmicb.2019.02829;
de Vall, é, e A, Bally P, Bruel C, Chandat L, Choquer M, Dieryckx C, Dupuy JW, Kaiser S, Latorse MP, Loisel E, Mey G, Morgant G, Rascle C, Schumacher J, Simon A, Souibgui E, Viaud M, Villalba F, Poussereau N, ATMT-Mutants? Front Microbiol, 10():2829(2019) [pubmed]
Keyword List
submitter keyword: Botrytis cinerea, quantitative proteome, non phytopathogenic mutant
Contact List
Nathalie Poussereau
contact affiliationEquipe de génomique fonctionnelle des champignons pathogènes des plantes. UMR 5240 Microbiologie Adaptation et Microbiologie. 14 impasse Pierre Baizet 69263 Lyon , Cedex 09. France
contact emailnathalie.poussereau@univ-lyon1.fr
lab head
Dupuy Jean-William
contact affiliationPlateforme Proteome Bordeaux
contact emailjean-william.dupuy@u-bordeaux.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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