PXD013359

PXD013359 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of several non pathogenic mutants from Botrytis cinerea.
Description	The grey mould fungus Botrytis cinerea is a typical necrotrophic fungus that can infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to find new important genes involved in the fungal virulence, a mutant library was generated by random insertional mutagenesis, using Agrobacterium tumefaciens mediated transformation. Ten mutants exhibiting total loss of virulence towards different host plants were considered. Their molecular characterization revealed a single T-DNA insertion in unique and different loci. Using a proteomics approach, the secretome of four of these strains was compared to that of the parental strain and a common profile of reduced lytic enzymes was recorded. Significant variations in this profile, notably proteases and hemicellulases deficiencies, were observed and validated by biochemical tests. They were also a hallmark of the remaining six non pathogenic strains, suggesting the importance of these secreted proteins in the infectious process.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_02:51:34.279.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Botrytis cinerea; NCBI TaxID: 40559;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-03 06:57:09	ID requested
⏵ 1	2021-11-03 02:51:35	announced

de Vall, é, e A, Bally P, Bruel C, Chandat L, Choquer M, Dieryckx C, Dupuy JW, Kaiser S, Latorse MP, Loisel E, Mey G, Morgant G, Rascle C, Schumacher J, Simon A, Souibgui E, Viaud M, Villalba F, Poussereau N, ATMT-Mutants? Front Microbiol, 10():2829(2019) [pubmed]

submitter keyword: Botrytis cinerea, non phytopathogenic mutant, quantitative proteome

Nathalie Poussereau
contact affiliation	Equipe de génomique fonctionnelle des champignons pathogènes des plantes. UMR 5240 Microbiologie Adaptation et Microbiologie. 14 impasse Pierre Baizet 69263 Lyon , Cedex 09. France
contact email	nathalie.poussereau@univ-lyon1.fr
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD013359

PRIDE project URI

• PRIDE
  1. PXD013359
     1. Label: PRIDE project
     2. Name: Proteomic analysis of several non pathogenic mutants from Botrytis cinerea.