PXD013358

PXD013358 is an original dataset announced via ProteomeXchange.

Title	Toxins from scratch? - Diverse, multimodal gene origins in the predatory robber fly Dasypogon diadema indicate dynamic venom evolution in dipteran insects
Description	Venoms and the toxins they contain represent molecular adaptations that have evolved on numerous occasions throughout the animal kingdom. However, the processes that shape venom protein evolution are poorly understood because of the scarcity of whole genome data available for comparative analyses of venomous species. Here, we perform a broad comparative toxicogenomic analysis to gain insight into the genomic mechanisms of venom evolution in robber flies (Asilidae). We first sequenced a high-quality draft genome of the hymenopteran hunting robber fly Dasypogon diadema, and analysed its venom by a combined proteotranscriptomic approach, and compared our results to recently described robber fly venoms to assess the general composition and major components of asilid venom. We then applied a comparative genomics approach, based on one additional asilid genome, ten high-quality dipteran genomes, and two lepidopteran outgroup-genomes, to reveal the evolutionary mechanisms and origins of identified venom proteins in robber flies. While some venom proteins were identified in the non-asilid genomes, several of the identified highly expressed venom proteins appear to be unique to robber flies. Our results reveal that the venom of D. diadema likely evolves in a multimodal fashion comprising 1) neofunctionalization after gene duplication, 2) expression-dependent co-option of proteins and 3) asilid lineage-specific orphan genes with enigmatic origin. The role of such orphan genes is currently being disputed in evolutionary genomics, but has not yet discussed in the context of toxin evolution. Our results display an unexpected dynamic venom evolution in asilid insects, which contrasts the findings of the only other insect toxicogenomic evolutionary analysis, in parasitoid wasps (Hymenoptera), were toxin evolution is dominated by single gene co-option.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:58:48.221.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eivind Undheim
SpeciesList	scientific name: Dasypogon diadema; NCBI TaxID: 468822;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-03 05:14:05	ID requested
1	2019-06-12 17:40:13	announced
2	2019-07-18 00:46:17	announced	Updated publication reference for PubMed record(s): 31289835.
⏵ 3	2024-10-22 03:58:56	announced	2024-10-22: Updated project metadata.

Drukewitz SH, Bokelmann L, Undheim EAB, von Reumont BM, Toxins from scratch? Diverse, multimodal gene origins in the predatory robber fly Dasypogon diadema indicate a dynamic venom evolution in dipteran insects. Gigascience, 8(7):(2019) [pubmed]

10.1093/gigascience/giz081;

submitter keyword: toxin, venom, Dasypogon,robber fly

Eivind Undheim
contact affiliation	Centre for Advanced Imaging, the University of Queensland
contact email	e.undheim@uq.edu.au
lab head
Eivind Undheim
contact affiliation	University of Queensland
contact email	eivindandreas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD013358

PRIDE project URI

• PRIDE
  1. PXD013358
     1. Label: PRIDE project
     2. Name: Toxins from scratch? - Diverse, multimodal gene origins in the predatory robber fly Dasypogon diadema indicate dynamic venom evolution in dipteran insects