PXD013340

PXD013340 is an original dataset announced via ProteomeXchange.

Title	Impact of increased core fucosylation on the exosome proteome in prostate cancer
Description	Prostate cancer is the most commonly diagnosed oncogenic malignancy in men worldwide, resulting in almost 30,000 cancer-related deaths in the United States each year. Wider examination of aberrant glycosylation in prostate cancer has revealed increased expression of the glycotransferase involved in core fucosylation, (1,6)fucosyltranferase (FUT8) associating with aggressive (AG) prostate cancer and castration-resistance, with functional analyses revealing FUT8 impacting cell motility and invasiveness in prostate cancer cells. Exosomes are extracellular microvesicles (30-150 nm) that are involved in in both proximal and distal intercellular communication via the transport of proteins and nucleic acids (mRNA, miRNA, and DNA). To gain insight into the impact of increased cellular FUT8 expression on exosome biogenesis and protein cargo profiles in prostate cancer, we paired Nanoparticle Tracking Analysis (NTA) and stable isotope labelling with amino acids in cell culture (SILAC) quantitative proteomics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:03:34.980.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Clark
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-02 03:59:03	ID requested
1	2020-05-12 22:21:23	announced
⏵ 2	2024-10-22 05:03:37	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.9b00578;

Clark DJ, Schnaubelt M, Hoti N, Hu Y, Zhou Y, Gooya M, Zhang H, Impact of Increased FUT8 Expression on the Extracellular Vesicle Proteome in Prostate Cancer Cells. J Proteome Res, 19(6):2195-2205(2020) [pubmed]

submitter keyword: Human, glycosylation, cancer, exosomes, glycoproteomics, proteomic

Hui Zhang
contact affiliation	Department of Pathology, The Johns Hopkins University, Baltimore, MD, USA
contact email	huizhang@jhu.edu
lab head
David Clark
contact affiliation	The Johns Hopkins University
contact email	dclark71@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD013340

PRIDE project URI

• PRIDE
  1. PXD013340
     1. Label: PRIDE project
     2. Name: Impact of increased core fucosylation on the exosome proteome in prostate cancer