PXD013339

PXD013339 is an original dataset announced via ProteomeXchange.

Title	Identification of HJURP binding determinants on the Mis18 complex using photo-cross-linking and mass spectrometry
Description	In the early G1 phase of the cell cycle, human HJURP is recruited to centromeres by the Mis18 complex, which consists of Mis18α, Mis18β and M18BP1. This interaction of HJURP with the Mis18 complex is essential for CENP-A deposition and the preservation of centromere identity. To identify the binding site of HJURP on the Mis18 complex, we used the amber-codon suppression method to incorporate unnatural photo-cross-linking amino acids (Bpa, p-benzoyl-L-phenylalanine; AbK, 3’-azibutyl-N-carbamoyl-lysine) and performed UV-cross-linking and mass spectrometry (MS) experiments.
HostingRepository	PRIDE
AnnounceDate	2019-08-01
AnnouncementXML	Submission_2019-08-01_06:03:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongqing Pan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-02 03:03:58	ID requested
⏵ 1	2019-08-01 06:03:20	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Photo-cross-linking, Bpa, AbK, StavroX, Q-Exactive, human, centromere, kinetochore, protein complex

Andrea Musacchio
contact affiliation	Max-Planck Institute for Molecular Physiology, Dortmund, Germany
contact email	andrea.musacchio@mpi-dortmund.mpg.de
lab head
Dongqing Pan
contact affiliation	Department of Mechanistic Cell Biology, Max-Planck Institute of Molecular Physiology, 9 Otto-Hahn-Str. 11, 44227 Dortmund
contact email	dongqing.pan@mpi-dortmund.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013339

PRIDE project URI

• PRIDE
  1. PXD013339
     1. Label: PRIDE project
     2. Name: Identification of HJURP binding determinants on the Mis18 complex using photo-cross-linking and mass spectrometry