PXD013327

PXD013327 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic alterations of human iPSC-based neuronal development indicate early onset of Rett syndrome.
Description	Rett syndrome (RTT) is a progressive neurodevelopmental disease often caused by mutations in the X-linked gene encoding methyl-CpG binding protein 2 (MeCP2). The mechanisms by which impaired MeCP2 induces the pathological abnormalities in the brain is not understood. To understand the molecular mechanisms involved in disease onset, we used an RTT patient induced pluripotent stem cell (iPSC)-based model and applied an in-depth high-resolution quantitative mass spectrometry-based analysis during early stages of neuronal development. Our data provided evidence of proteomic alteration at developmental stages long before the phase that symptoms of RTT syndrome become apparent. Differential expression increased from early to late neural stem cell phases, although proteins involved in immunity, metabolic processes and calcium signaling were already affected at initial stages. This data can help development of new biomarkers and therapeutic approaches in RTT syndrome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:25:54.662.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suzy Varderidou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-01 05:19:27	ID requested
1	2021-09-08 09:32:02	announced
⏵ 2	2024-10-22 05:25:58	announced	2024-10-22: Updated project metadata.

Varderidou-Minasian S, Hinz L, Hagemans D, Posthuma D, Altelaar M, Heine VM, Quantitative proteomic analysis of Rett iPSC-derived neuronal progenitors. Mol Autism, 11(1):38(2020) [pubmed]

10.1186/s13229-020-00344-3;

submitter keyword: Rett syndrome

iPSC

neuron differentiation

quantitative mass spectrometry

TMT-10plex

Maarten Altelaar
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, the Netherlands.
contact email	m.altelaar@uu.nl
lab head
Suzy Varderidou
contact affiliation	S
contact email	suzannavarderidou@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD013327

PRIDE project URI

• PRIDE
  1. PXD013327
     1. Label: PRIDE project
     2. Name: Quantitative proteomic alterations of human iPSC-based neuronal development indicate early onset of Rett syndrome.