PXD013326

PXD013326 is an original dataset announced via ProteomeXchange.

Title	Proteomics Analysis of Evolved Escherichia coli Populations from Cyclic Antibiotic Treatment
Description	When the survivors of antibiotic treatment (persisters) are repeatedly regrown and retreated with the same antibiotic for several cycles, the new population will soon adapt to the treatment condition and become tolerant to the drug. Here, we did evolution experiments on Escherichia coli populations by treating it with daily high concentration of different antibiotics (ampicillin, ciprofloxacin and apramycin) approximating clinical dosage, during the rapid growth-exponential phase. After a few cycles, we observed that the evolved populations exhibit extremely high tolerance to the drug, which are achieved by single point mutations in one of several genes. Interestingly, treatment with different antibiotics led to the selection of different mutants despite the shared persistence phenotype. Here, we applied spectral counting-based quantitative proteomics to study the proteome profile of the evolved E. coli populations from different cyclic antibiotic treatments.
HostingRepository	PRIDE
AnnounceDate	2020-01-10
AnnouncementXML	Submission_2020-01-17_06:29:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jordy Evan Sulaiman
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-01 05:19:23	ID requested
1	2020-01-10 05:52:22	announced
⏵ 2	2020-01-17 06:29:40	announced	2020-01-17: Updated publication reference for PubMed record(s): 31920087.

Sulaiman JE, Lam H, Populations from Cyclic Antibiotic Treatment. J Proteome Res, 19(2):900-913(2020) [pubmed]

submitter keyword: persisters, persistence, evolved populations, antibiotic, proteomics

Henry Lam
contact affiliation	Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST), Hong Kong
contact email	kehlam@ust.hk
lab head
Jordy Evan Sulaiman
contact affiliation	The Hong Kong University of Science and Technology (HKUST)
contact email	jesulaiman@connect.ust.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD013326

PRIDE project URI

• PRIDE
  1. PXD013326
     1. Label: PRIDE project
     2. Name: Proteomics Analysis of Evolved Escherichia coli Populations from Cyclic Antibiotic Treatment