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PXD013323

PXD013323 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleiTRAQ of Wucai (Brassica campestris L.)leaf color
DescriptionChlorophyll plays critical roles in photosynthetic light harvesting, energy transduction and plant development. In this study, a novel wucai (Brassica campestris L.) germplasm with green outer leaves and yellow inner leaves at the adult stage (W7-2) was used to examine chlorophyll metabolism response to cold acclimation. A green leaf wucai genotype without leaf color changes named W7-1 was selected as the control to evaluate the chlorophyll metabolism changes of W7-2. Compared to W7-1, the contents of chlorophyll a (Chl a) and chlorophyll b (Chl b) in W7-2 were significantly reduced at five developmental stages (13, 21, 29, 37 and 45 days after planting (DAP). An iTRAQ-based quantitative proteomic analysis was carried out at 21 and 29 DAP according to the leaf color changes in both of genotypes. A total of 1409 proteins were identified, of which 218 showed differential accumulations between W7-2 and W7-1 during the two developmental stages.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_03:59:15.294.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLingyun Yuan
SpeciesList scientific name: Brassica napus (Rape); NCBI TaxID: 3708;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-01 03:47:56ID requested
12019-05-03 14:08:24announced
22024-10-22 03:59:23announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.jproteome.9b00016;
Xie S, Nie L, Zheng Y, Wang J, Zhao M, Zhu S, Hou J, Chen G, Wang C, Yuan L, Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai ( Brassica campestris L.) Responding to Cold Acclimation. J Proteome Res, 18(6):2478-2492(2019) [pubmed]
Keyword List
submitter keyword: Wucai
Leaf color
iTRAQ-based quantitative proteomics
Chlorophyll biosynthesis
Contact List
Lingyun Yuan
contact affiliationCollege of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, 230036 Hefei, Anhui, China;
contact emailylyun99@163.com
lab head
Lingyun Yuan
contact affiliationAnhui Agricultural University
contact emailylyun99@163.com
dataset submitter
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Dataset FTP location
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