PXD013315

PXD013315 is an original dataset announced via ProteomeXchange.

Title	Membrane proteomic analysis reveals the intestinal development is deteriorated by intra-uterine growth restriction in piglets
Description	The alterations of the intestinal proteome was observed in intrauterine growth restriction (IUGR) piglets during early life by gel-based approaches. Nevertheless, how IUGR affects the intestinal membrane proteome during neonatal development remains unclear. Here, we applied the iTRAQ-based proteomics technology and biochemical analysis to investigate the impact of IUGR on the membrane proteome of the jejunal mucosa in the piglets. 361 membrane proteins were screened by functional prediction. Among them, eight, five and one differentially expressed membrane proteins were identified between IUGR and NBW piglets at day 0, day 7 and day 21 after birth, respectively. Differentially expressed membrane proteins (DEMPs) including F1SBL3, F1RRW8, F1S539, F1S2Z2, F1RIR2, F1RUF2 I3LP60, Q2EN79 and F1SIH8 were reduced while the relative abundance of I3L6A2, F1SCJ1, F1RI18, I3LRJ7 and F1RNN0 were increased in IUGR piglets than NBW piglets. From the aspects of function, F1RRW8, F1S539, F1S2Z2 and F1RIR2 are mainly associated with D2 dopamine receptor binding, transmembrane transport of small molecules, signal transduction and translocation of GLUT4, respectively, and F1SIH8, I3LRJ7 and F1RNN0 are related to autophagy, metabolism of vitamins and intracellular protein transport. Additionally, IUGR decreased the level of proteins (F1RRW8, Q2EN79 and F1RI18) that are involved in response to oxidative stress.
HostingRepository	iProX
AnnounceDate	2019-03-29
AnnouncementXML	Submission_2023-08-27_21:08:39.516.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shimeng Huang
SpeciesList	scientific name: Sus scrofa domesticus; NCBI TaxID: 9825;
ModificationList	iTRAQ8plex reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-31 23:54:17	ID requested
⏵ 1	2023-08-27 21:08:40	announced

Huang S, Liu C, Li N, Wu Z, Li T, Han D, Li Z, Zhao J, Wang J, Membrane proteomic analysis reveals the intestinal development is deteriorated by intrauterine growth restriction in piglets. Funct Integr Genomics, 20(2):277-291(2020) [pubmed]

submitter keyword: iTRAQ, intrauterine growth restriction, neonatal piglets, membrane proteome, intestine

Junjun Wang
contact affiliation	State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University
contact email	jkywjj@hotmail.com
lab head
Shimeng Huang
contact affiliation	State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University
contact email	shimengh@hotmail.com
dataset submitter

iProX dataset URI