PXD013310

PXD013310 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of simian primary hepatocytes reveals candidate molecular markers for permissiveness to relapsing malaria Plasmodium cynomolgi
Description	A major obstacle in deciphering the hepatic stage of the malaria parasite has been the challenges associated with culturing the infected hepatocytes through the entire liver stage cycle, including that of the dormant form known as hypnozoites. Primary hepatocytes lose their specialized functions in long-term in vitro culture. Hepatocyte infection represents the first step for clinically silent infection and development of malaria parasite Plasmodium in the liver. Thus this liver stage is an ideal target for development of novel antimalarial drugs and vaccine. However, drug discovery against Plasmodium liver stage is severely hampered by the poor understanding of host-cell and parasites interactions during the liver stage infection and development. In this study, we have performed tandem mass tags (TMT) labelling based quantitative proteomic analysis in simian primary hepatocytes cultured in three different systems of susceptibility to plasmodium infection. Our results represent the first documentation of potentially essential molecular markers including asialoglycoprotein receptor (ASGPR), apolipoproteins, squalene synthase and scavenger receptor B1 (SR-BI) required for productive infection and full development in relapsing Plasmodium species. The identification of these candidate proteins for constructive infection and development of Plasmodium in malaria paves the way to explore them as therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2019-09-02
AnnouncementXML	Submission_2019-09-02_01:04:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bamaprasad Dutta
SpeciesList	scientific name: Macaca fascicularis; NCBI TaxID: 9541;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-29 06:52:31	ID requested
⏵ 1	2019-09-02 01:04:26	announced

Dembele L, Gupta DK, Dutta B, Chua ACY, Sze SK, Bifani P, cynomolgi. Proteomics, 19(19):e1900021(2019) [pubmed]

submitter keyword: Quantitative proteomic, LC-MS/MS, TMT, Malaria liver stage, Drug assay model development, Biomarker, permissiveness to infection

Siu Kwan SZE
contact affiliation	School of Biological Sciences Nanyang Technological University 60 Nanyang Drive, Singapore 637551
contact email	sksze@ntu.edu.sg
lab head
Bamaprasad Dutta
contact affiliation	Nanyang Technological University
contact email	bama0001@e.ntu.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD013310

PRIDE project URI

• PRIDE
  1. PXD013310
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of simian primary hepatocytes reveals candidate molecular markers for permissiveness to relapsing malaria Plasmodium cynomolgi