PXD013304

PXD013304 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	VEX1-GFP Pull-downs
Description	We affinity-purified VEX1GFP-associated proteins using cryomilling and high-affinity nanobodies. The procedure was carried out initially in insect-stage T. brucei, for which protocols were established, and then in bloodstream-form T. brucei. Quantitative proteomic analysis revealed tag-dependent enrichment of GFP and the same set of five proteins in four independent experiments; VEX1 was enriched, as expected, but also Tb927.11.13380, an ortholog of the nonsense-mediated mRNA-decay (NMD) ATP-dependent superfamily 1-type helicases, UPF1/SMG2/NAM7/Rent1 19), now designated VEX2 (predicted 224-kDa, 2026 residues). The other three proteins that displayed tag-dependent enrichment were all three components of chromatin assembly factor 1 (CAF-1; CAF-1a, Tb927.8.3980; CAF-1b, Tb927.10.7050, CAF-1c, Tb927.11.4970a), the evolutionary conserved hetero-trimeric replication-associated histone chaperone.
HostingRepository	PRIDE
AnnounceDate	2019-05-31
AnnouncementXML	Submission_2019-05-31_03:22:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013304
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joana Faria
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	Oxidation; Dioxidation; Carbamidomethyl; Acetyl; Gln->pyro-Glu
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-29 04:53:38	ID requested
⏵ 1	2019-05-31 03:22:01	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: VEX1
VSG
allelic exclusion
Trypanosoma brucei

submitter keyword: VEX1

VSG

allelic exclusion

Trypanosoma brucei

Contact List

David Horn
contact affiliation	Professor of Parasite Molecular Biology Biological Chemistry and Drug Discovery Wellcome Centre for Anti-Infectives Research (WCAIR) School of Life Sciences, University of Dundee
contact email	d.horn@dundee.ac.uk
lab head
Joana Faria
contact affiliation	Wellcome Trust Centre for Anti-infectives Research, School of Life Sciences, University of Dundee
contact email	jrcorreiafaria@dundee.ac.uk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD013304

PRIDE project URI

Repository Record List

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