PXD013293

PXD013293 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Rapid discovery of drug target engagement by isothermal shift assay
Description	Determining protein targets directly bound by drug molecules remains challenging. Here we present the isothermal shift assay, iTSA, for rapid unbiased identification of drug targets. Compared with thermal proteome profiling, the prevailing method for target engagement, iTSA offers a simplified workflow, 4- fold higher throughput, and a multiplexed experimental design with higher replication. We demonstrate its application to determination of target engagement for several kinase inhibitors in lysates and living cells.
HostingRepository	MassIVE
AnnounceDate	2019-03-29
AnnouncementXML	Submission_2019-03-29_09:16:40.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	William Old
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; Acetyl
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-28 10:27:44	ID requested
⏵ 1	2019-03-29 09:16:40	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: iTSA, proteome, thermal shift

submitter keyword: iTSA, proteome, thermal shift

Contact List

William Old
contact affiliation	University of Colorado Boulder
contact email	William.Old@colorado.edu
lab head
William Old
contact affiliation	University of Colorado, Boulder
contact email	William.Old@colorado.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083640