PXD013284

PXD013284 is an original dataset announced via ProteomeXchange.

Title	HiRIEF-LC-MS based proteomics analysis of subcellular fractions of human CD4+ T-Cells with and without T-cell Receptor stimulation.
Description	Subcellular proteomics analysis of human T-cells in response to T-cell receptor stimulation. High Resolution Isoelectric Focusing (HiRIEF) LC-MS/MS and relative quantification by TMT 10-plex was used to analyze subcellular fractions (Cytosol, Nuclear and Mitochondria-Small Organelle-Membrane) of Human T-cells from 3 separate donors stimulated with T-cell receptor stimulation for 0, 15 minutes or 60 minutes. The data was obtained from 3 separate TMT10 plex sets which included: 126 – Cytosol, unstimulated; 127N – Cyto, 15 min; 127C – Cyto, 60 min; 128N – Mitochondrion-Small Organelle-Membrane, unstimulated; 128C – Mito-SmO-Mem, 15 min; 129N – Mito-SmO-Mem, 60 min; 129C – Nuclear, unstimulated; 130N – Nuc, 15 min; 130C– Nuc, 60 min; 131 – pooled internal control of all samples.
HostingRepository	PRIDE
AnnounceDate	2019-11-13
AnnouncementXML	Submission_2020-01-07_06:57:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mattias Vesterlund
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-28 06:47:14	ID requested
1	2019-11-13 04:09:37	announced
⏵ 2	2020-01-07 06:57:32	announced	2020-01-07: Updated publication reference for PubMed record(s): 31849937.

Joshi RN, Stadler C, Lehmann R, Lehti, ö J, Tegn, é, r J, Schmidt A, Vesterlund M, TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells. Front Immunol, 10():2708(2019) [pubmed]

submitter keyword: T-cell, Human, Subcellular, T-cell receptor

Janne Lehtiö
contact affiliation	Dept. Oncology Pathology, Karolinska Institutet, and Scilifelab, Stockholm, Sweden
contact email	janne.lehtio@ki.se
lab head
Mattias Vesterlund
contact affiliation	Karolinska Institutet
contact email	mattias.vesterlund@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD013284

PRIDE project URI

• PRIDE
  1. PXD013284
     1. Label: PRIDE project
     2. Name: HiRIEF-LC-MS based proteomics analysis of subcellular fractions of human CD4+ T-Cells with and without T-cell Receptor stimulation.