PXD013270

PXD013270 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of adipose tissue derived Exosome-like vesicles
Description	Scope: Adipose tissue is regarded as a true endocrine organ. Recent studies showed that adipose tissue derived exosomes could serve as carrier of circulating miRNAs to regulate distant targets. However, the characteristics of exosomal proteins released from adipose tissue have not been investigated yet. Methods and Results: In this study, we conducted a complementary protein profiling on exosome-like vesicles derived from adipose tissue (ELV-AT) with Label-free Quantitative Proteomic Analysis. A total of 3229 ELV-AT proteins were identified, among which only 266 proteins have been annotated as adipokines. 3 undefined adipokine candidates (NPM3, STEAP3, and DAD1) were selected for further validation. These 3 proteins were expressed in both white and brown adipose tissue, upregulated during adipogenic differentiation of both 3T3-L1 cells and adipose derived stem cells (ADSCs). Expressions of NPM3, DAD1 in ELV-AT were significantly decreased in obese subjects compared with lean controls while obesity could not alter the expression of STEAP3. Conclusions: Our profiling study of the ELV-AT proteins expands the list of adipokines and highlights the pivotal role of exosomal adipokines in the regulation of multiple biological processes within adipose tissue
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:08:17.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YAN ZHAGN
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-27 04:32:29	ID requested
1	2020-07-02 02:09:25	announced
⏵ 2	2024-10-22 05:08:20	announced	2024-10-22: Updated project metadata.

Zhang Y, Yu M, Dong J, Wu Y, Tian W, Identification of Novel Adipokines through Proteomic Profiling of Small Extracellular Vesicles Derived from Adipose Tissue. J Proteome Res, 19(8):3130-3142(2020) [pubmed]

10.1021/acs.jproteome.0c00131;

submitter keyword: exosome-like vesicles, obesity,adipokines, adipose tissue

Weidong Tian
contact affiliation	State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Oral Regenerative Medicine & Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University
contact email	drtwd@sina.com
lab head
YAN ZHAGN
contact affiliation	Sichuan University
contact email	dentistzy@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD013270

PRIDE project URI

• PRIDE
  1. PXD013270
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of adipose tissue derived Exosome-like vesicles